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Title: Biological responsiveness to the phorbol esters and specific binding of (/sup 3/H)phorbol 12,13-dibutyrate in the nematode Caenorhabditis elegans, a manipulable genetic system

Because of its suitability for genetic studies, the nematode Caenorhabditis elegans was examined for its responsiveness to the phorbol esters. Phorbol 12-myristate 13-acetate had three effects. It inhibited the increase in animal size during growth; it decreased the yield of progeny; and it caused uncoordinated movement of the adult. The effects on nematode size, progeny yield, and movement were quantitated. Concentrations of phorbol 12-myristate 13-acetate yielding half-maximal responses were 440, 460, and 170 nM, respectively. As was expected from the biological responsiveness of the nematodes, specific, saturable binding of phorbol ester to nematode extracts was found. (/sup 3/H)phorbol 12,13-dibutyrate bound with a dissociation constant of 26.8 +/- 3.9 nM. At saturation, 5.7 +/- 1.4 pmole/mg protein was bound.
Authors:
; ;
Publication Date:
OSTI Identifier:
6841422
Resource Type:
Journal Article
Resource Relation:
Journal Name: Teratog., Carcinog., Mutagen.; (United States); Journal Volume: 2:1
Research Org:
Department of Neuropathology, Harvard Medical School, Boston
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; NEMATODES; SENSITIVITY; PHORBOL ESTERS; TOXICITY; TRITIUM COMPOUNDS; TRACER TECHNIQUES; ANIMAL GROWTH; BEHAVIOR; BIOCHEMICAL REACTION KINETICS; DOSE-RESPONSE RELATIONSHIPS; GENETICS; PROGENY; ASCHELMINTHES; BIOLOGY; CARCINOGENS; ESTERS; GROWTH; HELMINTHS; ISOTOPE APPLICATIONS; KINETICS; LABELLED COMPOUNDS; ORGANIC COMPOUNDS; REACTION KINETICS 560304* -- Chemicals Metabolism & Toxicology-- Invertebrates-- (-1987); 550201 -- Biochemistry-- Tracer Techniques