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Title: cap alpha. /sub i/-3 cDNA encodes the. cap alpha. subunit of G/sub k/, the stimulatory G protein of receptor-regulated K/sup +/ channels

Abstract

cDNA cloning has identified the presence in the human genome of three genes encoding ..cap alpha.. subunits of pertussis toxin substrates, generically called G/sub i/. They are named ..cap alpha../sub i/-1, ..cap alpha../sub i/-2 and ..cap alpha../sub i/-3. However, none of these genes has been functionally identified with any of the ..cap alpha.. subunits of several possible G proteins, including pertussis toxin-sensitive G/sub p/'s, stimulatory to phospholipase C or A/sub 2/, G/sub i/, inhibitory to adenylyl cyclase, or G/sub k/, stimulatory to a type of K/sup +/ channels. The authors now report the nucleotide sequence and the complete predicted amino acid sequence of human liver ..cap alpha../sub i/-3 and the partial amino acid sequence of proteolytic fragments of the ..cap alpha.. subunit of human erythrocyte G/sub k/. The amino acid sequence of the proteolytic fragment is uniquely encoded by the cDNA of ..cap alpha../sub i/-3, thus identifying it as ..cap alpha../sub k/. The probable identity of ..cap alpha../sub i/-1 with ..cap alpha../sub p/ and possible roles for ..cap alpha../sub i/-2, as well as additional roles for ..cap alpha../sub i/-1 and ..cap alpha../sub i/-3 (..cap alpha../sub k/) are discussed.

Authors:
; ; ; ; ;
Publication Date:
Research Org.:
Baylor College of Medicine, Houston, TX (USA)
OSTI Identifier:
6838327
Resource Type:
Journal Article
Journal Name:
J. Biol. Chem.; (United States)
Additional Journal Information:
Journal Volume: 263:14
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; RECOMBINANT DNA; DNA SEQUENCING; DNA-CLONING; TOXINS; AMINO ACID SEQUENCE; ATP; CATIONS; ERYTHROCYTES; GENES; LIVER; MAN; MEMBRANE TRANSPORT; PHOSPHORUS 32; POTASSIUM COMPOUNDS; PROTEINS; RECEPTORS; SULFUR 35; ALKALI METAL COMPOUNDS; ANIMALS; ANTIGENS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY; BODY FLUIDS; CHARGED PARTICLES; CLONING; DAYS LIVING RADIOISOTOPES; DIGESTIVE SYSTEM; DNA; EVEN-ODD NUCLEI; GLANDS; IONS; ISOTOPES; LIGHT NUCLEI; MAMMALS; MATERIALS; MEMBRANE PROTEINS; MOLECULAR STRUCTURE; NUCLEI; NUCLEIC ACIDS; NUCLEOTIDES; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; ORGANS; PHOSPHORUS ISOTOPES; PRIMATES; RADIOISOTOPES; STRUCTURAL CHEMICAL ANALYSIS; SULFUR ISOTOPES; TOXIC MATERIALS; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Codina, J, Olate, J, Abramowitz, J, Mattera, R, Cook, R G, and Birnbaumer, L. cap alpha. /sub i/-3 cDNA encodes the. cap alpha. subunit of G/sub k/, the stimulatory G protein of receptor-regulated K/sup +/ channels. United States: N. p., 1988. Web.
Codina, J, Olate, J, Abramowitz, J, Mattera, R, Cook, R G, & Birnbaumer, L. cap alpha. /sub i/-3 cDNA encodes the. cap alpha. subunit of G/sub k/, the stimulatory G protein of receptor-regulated K/sup +/ channels. United States.
Codina, J, Olate, J, Abramowitz, J, Mattera, R, Cook, R G, and Birnbaumer, L. 1988. "cap alpha. /sub i/-3 cDNA encodes the. cap alpha. subunit of G/sub k/, the stimulatory G protein of receptor-regulated K/sup +/ channels". United States.
@article{osti_6838327,
title = {cap alpha. /sub i/-3 cDNA encodes the. cap alpha. subunit of G/sub k/, the stimulatory G protein of receptor-regulated K/sup +/ channels},
author = {Codina, J and Olate, J and Abramowitz, J and Mattera, R and Cook, R G and Birnbaumer, L},
abstractNote = {cDNA cloning has identified the presence in the human genome of three genes encoding ..cap alpha.. subunits of pertussis toxin substrates, generically called G/sub i/. They are named ..cap alpha../sub i/-1, ..cap alpha../sub i/-2 and ..cap alpha../sub i/-3. However, none of these genes has been functionally identified with any of the ..cap alpha.. subunits of several possible G proteins, including pertussis toxin-sensitive G/sub p/'s, stimulatory to phospholipase C or A/sub 2/, G/sub i/, inhibitory to adenylyl cyclase, or G/sub k/, stimulatory to a type of K/sup +/ channels. The authors now report the nucleotide sequence and the complete predicted amino acid sequence of human liver ..cap alpha../sub i/-3 and the partial amino acid sequence of proteolytic fragments of the ..cap alpha.. subunit of human erythrocyte G/sub k/. The amino acid sequence of the proteolytic fragment is uniquely encoded by the cDNA of ..cap alpha../sub i/-3, thus identifying it as ..cap alpha../sub k/. The probable identity of ..cap alpha../sub i/-1 with ..cap alpha../sub p/ and possible roles for ..cap alpha../sub i/-2, as well as additional roles for ..cap alpha../sub i/-1 and ..cap alpha../sub i/-3 (..cap alpha../sub k/) are discussed.},
doi = {},
url = {https://www.osti.gov/biblio/6838327}, journal = {J. Biol. Chem.; (United States)},
number = ,
volume = 263:14,
place = {United States},
year = {Sun May 15 00:00:00 EDT 1988},
month = {Sun May 15 00:00:00 EDT 1988}
}