Regulation of expression of an auxin-induced soybean sequence by cadmium
An auxin-regulated soybean sequence has been characterized and shown to be induced by the heavy metals cadmium, silver, and copper. Cadmium induces the accumulation of two size classes of mRNA: a 1-kilobase (kb) RNA class, which is the same size as the RNA class induced by auxin, silver, and copper, and a 1.4-kb RNA class. DNA sequences analysis of cDNA clones and a soybean genomic fragment has shown the presence of an intron in this gene. A restriction fragment probe isolated from the intron segment hybridizes specifically to the 1.4-kb mRNA. The transcription rate of this sequences is rapidly increased following exposure of soybean primary leaves to cadmium, as assayed by nuclear run-off transcription experiments. These results suggest that cadmium not only induces the transcription of a specific soybean sequences, but interferes with the processing of the precursor mRNA, resulting in the accumulation of the 1.4-kb mRNA precursor species.
- Research Organization:
- Univ. of Missouri, Columbia (USA)
- OSTI ID:
- 6834659
- Journal Information:
- J. Biol. Chem.; (United States), Vol. 263:13
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
CADMIUM COMPOUNDS
BIOLOGICAL EFFECTS
RECOMBINANT DNA
DNA SEQUENCING
AUXINS
COPPER COMPOUNDS
GENE REGULATION
MESSENGER-RNA
PHOSPHORUS 32
SILVER COMPOUNDS
SOYBEANS
TRANSCRIPTION
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
DNA
FOOD
ISOTOPES
LIGHT NUCLEI
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
PLANT GROWTH REGULATORS
PLANTS
RADIOISOTOPES
RNA
STRUCTURAL CHEMICAL ANALYSIS
TRANSITION ELEMENT COMPOUNDS
VEGETABLES
560300* - Chemicals Metabolism & Toxicology
550201 - Biochemistry- Tracer Techniques