Luminal and basolateral uptake of insulin in isolated perfused, proximal tubules

Nielsen, S; Nielsen, J T; Christensen, E I

Title: Luminal and basolateral uptake of insulin in isolated perfused, proximal tubules

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Abstract

The present study was performed to quantitate compare the luminal and the peritubular uptake of {sup 125}I-insulin in isolated, perfused, proximal tubules from rabbit kidneys. {sup 125}I-insulin was added in physiological concentrations to either the perfusate or the bath fluid for 30 min. The luminal uptake in 30 min averaged 0.76 pg/mm at physiological concentrations and 18.0 pg/mm at high insulin concentrations. About 15-41% of the absorbed insulin was digested and <5% was transported from the lumen to the peritubular space as intact insulin. The peritubular binding/uptake of {sup 125}I-insulin at physiological and high concentrations in the bath was 0.136 and 0.318 pg, respectively. The data indicates that insulin is bound/absorbed at the basolateral membranes both by a saturable specific mechanism and a nonspecific, nonsaturable mechanism. The basolateral absorption constituted 15.2 and 1.8% of the total tubular extraction of insulin at physiological and high insulin concentrations, respectively. Electron microscope autoradiography showed that, after luminal as well as basolateral endocytosis, insulin was exclusively accumulated in endocytic vacuoles and lysosomes.

Authors:

Nielsen, S; Nielsen, J T; Christensen, E I ^[1]

Univ. of Aarhus (Denmark)

Publication Date:: Sun Nov 01 00:00:00 EST 1987

OSTI Identifier:: 6834217

Resource Type:: Journal Article

Journal Name:: American Journal of Physiology; (USA)

Additional Journal Information:: Journal Volume: 253:5; Journal ID: ISSN 0002-9513

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; INSULIN; UPTAKE; LABELLED COMPOUNDS; TUBULES; AUTORADIOGRAPHY; CARBON 14 COMPOUNDS; INULIN; IODINE 125; KIDNEYS; RABBITS; TRANSMISSION ELECTRON MICROSCOPY; ANIMALS; BETA DECAY RADIOISOTOPES; BODY; CARBOHYDRATES; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; ELECTRON MICROSCOPY; HORMONES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPES; MAMMALS; MICROSCOPY; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANS; PEPTIDE HORMONES; POLYSACCHARIDES; RADIOISOTOPES; SACCHARIDES; VERTEBRATES; 551001* - Physiological Systems- Tracer Techniques

Citation Formats


                    Nielsen, S, Nielsen, J T, and Christensen, E I. Luminal and basolateral uptake of insulin in isolated perfused, proximal tubules.  United States: N. p., 1987. 
        Web.

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                    Nielsen, S, Nielsen, J T, & Christensen, E I. Luminal and basolateral uptake of insulin in isolated perfused, proximal tubules.  United States.

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                    Nielsen, S, Nielsen, J T, and Christensen, E I. 1987.  
        "Luminal and basolateral uptake of insulin in isolated perfused, proximal tubules".  United States.

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@article{osti_6834217,

  title        = {Luminal and basolateral uptake of insulin in isolated perfused, proximal tubules},

  author       = {Nielsen, S and Nielsen, J T and Christensen, E I},

  abstractNote = {The present study was performed to quantitate compare the luminal and the peritubular uptake of {sup 125}I-insulin in isolated, perfused, proximal tubules from rabbit kidneys. {sup 125}I-insulin was added in physiological concentrations to either the perfusate or the bath fluid for 30 min. The luminal uptake in 30 min averaged 0.76 pg/mm at physiological concentrations and 18.0 pg/mm at high insulin concentrations. About 15-41% of the absorbed insulin was digested and <5% was transported from the lumen to the peritubular space as intact insulin. The peritubular binding/uptake of {sup 125}I-insulin at physiological and high concentrations in the bath was 0.136 and 0.318 pg, respectively. The data indicates that insulin is bound/absorbed at the basolateral membranes both by a saturable specific mechanism and a nonspecific, nonsaturable mechanism. The basolateral absorption constituted 15.2 and 1.8% of the total tubular extraction of insulin at physiological and high insulin concentrations, respectively. Electron microscope autoradiography showed that, after luminal as well as basolateral endocytosis, insulin was exclusively accumulated in endocytic vacuoles and lysosomes.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/6834217},
  journal      = {American Journal of Physiology; (USA)},

  issn         = {0002-9513},

  number       = ,

  volume       = 253:5,

  place        = {United States},

  year         = {Sun Nov 01 00:00:00 EST 1987},

  month        = {Sun Nov 01 00:00:00 EST 1987}

}

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