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Title: Proliferation and differentiation of brown adipocytes from interstitial cells during cold acclimation

Abstract

The mechanisms of brown adipocyte proliferation and differentiation during cold acclimation (and/or adaptation to hyperphagia) have been studied by quantitative photonic radioautography. (/sup 3/H)thymidine was injected to warm-acclimated (25/sup 0/C) rats and to animals exposed to 5/sup 0/C for 2 days. Samples of interscapular brown adipose tissue were collected for quantitative analysis of mitotic frequencies at various periods of time (4 h-15 days) after the injection of (/sup 3/H)thymidine, the rats being maintained at the temperatures to which they were initially exposed. It was found that cold exposure for 2 days markedly enhanced mitotic activity in endothelial cells, interstitial cells, and brown preadipocytes rather than in fully differentiated brown adipocytes. The total tissue labeling index (percent of labeled nuclei) increased approx.70 times over control values. The authors now report that cellular labeling progressively increased in mature brown adipocytes during cold acclimation, whereas it correspondingly decreased in interstitial cells and brown preadipocytes. This indicates that the sequence of events for cellular differentiation is interstitial cells ..-->.. brown preadipocytes ..-->.. mature brown adipocytes. Remarkable, labeling frequency did not change in endothelial cells during cold acclimation demonstrating that these cells cannot be considered as progenitors of brown adipocytes. It is suggested that brownmore » adipocyte proliferation and differentiation from interstitial cells represent the fundamental phenomena explaining the enhanced capacity of cold-acclimated and/or hyperphagic rats to respond calorigenically to catecholamines.« less

Authors:
; ;
Publication Date:
Research Org.:
Laval Univ., Quebec
OSTI Identifier:
6831668
Resource Type:
Journal Article
Journal Name:
Am. J. Physiol.; (United States)
Additional Journal Information:
Journal Volume: 250:6
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ADIPOSE TISSUE; AUTORADIOGRAPHY; FAT CELLS; CELL DIFFERENTIATION; CELL PROLIFERATION; TEMPERATURE EFFECTS; BIOLOGICAL ADAPTATION; CATECHOLAMINES; MITOSIS; RATS; THYMIDINE; TRITIUM COMPOUNDS; AMINES; ANIMAL CELLS; ANIMAL TISSUES; ANIMALS; AROMATICS; AZINES; BODY; CELL DIVISION; CONNECTIVE TISSUE; CONNECTIVE TISSUE CELLS; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; LABELLED COMPOUNDS; MAMMALS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PHENOLS; POLYPHENOLS; PYRIMIDINES; RIBOSIDES; RODENTS; SOMATIC CELLS; TISSUES; VERTEBRATES; 550301* - Cytology- Tracer Techniques

Citation Formats

Bukowiecki, L J, Geloeen, A, and Collet, A J. Proliferation and differentiation of brown adipocytes from interstitial cells during cold acclimation. United States: N. p., 1986. Web.
Bukowiecki, L J, Geloeen, A, & Collet, A J. Proliferation and differentiation of brown adipocytes from interstitial cells during cold acclimation. United States.
Bukowiecki, L J, Geloeen, A, and Collet, A J. 1986. "Proliferation and differentiation of brown adipocytes from interstitial cells during cold acclimation". United States.
@article{osti_6831668,
title = {Proliferation and differentiation of brown adipocytes from interstitial cells during cold acclimation},
author = {Bukowiecki, L J and Geloeen, A and Collet, A J},
abstractNote = {The mechanisms of brown adipocyte proliferation and differentiation during cold acclimation (and/or adaptation to hyperphagia) have been studied by quantitative photonic radioautography. (/sup 3/H)thymidine was injected to warm-acclimated (25/sup 0/C) rats and to animals exposed to 5/sup 0/C for 2 days. Samples of interscapular brown adipose tissue were collected for quantitative analysis of mitotic frequencies at various periods of time (4 h-15 days) after the injection of (/sup 3/H)thymidine, the rats being maintained at the temperatures to which they were initially exposed. It was found that cold exposure for 2 days markedly enhanced mitotic activity in endothelial cells, interstitial cells, and brown preadipocytes rather than in fully differentiated brown adipocytes. The total tissue labeling index (percent of labeled nuclei) increased approx.70 times over control values. The authors now report that cellular labeling progressively increased in mature brown adipocytes during cold acclimation, whereas it correspondingly decreased in interstitial cells and brown preadipocytes. This indicates that the sequence of events for cellular differentiation is interstitial cells ..-->.. brown preadipocytes ..-->.. mature brown adipocytes. Remarkable, labeling frequency did not change in endothelial cells during cold acclimation demonstrating that these cells cannot be considered as progenitors of brown adipocytes. It is suggested that brown adipocyte proliferation and differentiation from interstitial cells represent the fundamental phenomena explaining the enhanced capacity of cold-acclimated and/or hyperphagic rats to respond calorigenically to catecholamines.},
doi = {},
url = {https://www.osti.gov/biblio/6831668}, journal = {Am. J. Physiol.; (United States)},
number = ,
volume = 250:6,
place = {United States},
year = {Sun Jun 01 00:00:00 EDT 1986},
month = {Sun Jun 01 00:00:00 EDT 1986}
}