Fluorometric determination of hydrogen peroxide in groundwater
The fluorometric scopoletin-horseradish peroxidase method has been modified for field determinations of hydrogen peroxide concentrations in groundwaters. Standard additions calibration compensates for background fluorescence and inconsistent stoichiometry of the fluorescence quenching reaction due to interferences by the matrix. The detection limit, defined as the blank plus three standard deviations, ranged from 3.6 to 44.6 nM. However, this limit was more an indication of the difficulty of preparing peroxide-free water than the actual limit imposed by the sensitivity of the method for the peroxide contamination introduced with the reagents. For 111 field determinations the weighted average (uncorrected) hydrogen peroxide concentration was 20.2 nM and the pooled standard deviation was 7.7 nM. The average of 45 field blanks was 7.8 nM with a pooled standard deviation of 5.2 nM. At nanomolar concentration levels, it is essential that samples are analyzed for H/sub 2/O/sub 2/ in the field. Storage periods exceeding 1 h caused serious errors and irreproducible results.
- Research Organization:
- Illinois Dept. of Energy and Natural Resources, Champaign
- OSTI ID:
- 6814210
- Journal Information:
- Anal. Chem.; (United States), Vol. 59:4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
54 ENVIRONMENTAL SCIENCES
GROUND WATER
QUANTITATIVE CHEMICAL ANALYSIS
HYDROGEN PEROXIDE
FLUORESCENCE SPECTROSCOPY
EXPERIMENTAL DATA
PEROXIDASES
REDOX POTENTIAL
REDOX REACTIONS
TRACE AMOUNTS
WATER CHEMISTRY
CHEMICAL ANALYSIS
CHEMICAL REACTIONS
CHEMISTRY
DATA
EMISSION SPECTROSCOPY
ENZYMES
HYDROGEN COMPOUNDS
INFORMATION
NUMERICAL DATA
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PEROXIDES
SPECTROSCOPY
WATER
400102* - Chemical & Spectral Procedures
520200 - Environment
Aquatic- Chemicals Monitoring & Transport- (-1989)