Preparation of stable sup 125 I cyclic GMP tyrosine methyl ester suitable for 3',5' cyclic GMP radioimmunoassay by HPLC

Journal Article · Mon Jan 01 00:00:00 EST 1990 · Journal of Immunoassay; (USA)

DOI:https://doi.org/10.1080/01971529008053261· OSTI ID:6809616

Thompson, M R; Luttrell, M; Giannella, R A ^[1]

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1. Univ. of Cincinnati College of Medicine, OH (USA)

Determination of the concentration of cyclic guanosine monophosphate (cGMP) by radioimmunoassay (RIA) depends upon the availability of suitable radiolabeled tracers and antibody to detect the product. Reverse phase chromatographic techniques can easily separate the reaction products of chloramine-T iodination of succinyl cGMP tyrosine methyl ester. The binding characteristics of the iodination reaction products to anti-cGMP antibody have been determined. Purified succinyl cyclic nucleotide 125I-tyrosine methyl ester binds to cGMP antisera identically as commercially available tracer. The tracer is stable for greater than three months.

• OSTI ID: 6809616
• Journal Information: Journal of Immunoassay; (USA), Vol. 11:1; ISSN 0197-1522
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
NUCLEOTIDES
RADIOIMMUNOASSAY
CHEMICAL PREPARATION
GUANOSINE
IODINATION
IODINE 125
LABELLING
LIQUID COLUMN CHROMATOGRAPHY
TYROSINE
AMINO ACIDS
AROMATICS
AZAARENES
BETA DECAY RADIOISOTOPES
BIOASSAY
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
ELECTRON CAPTURE RADIOISOTOPES
HALOGENATION
HETEROCYCLIC COMPOUNDS
HYDROXY ACIDS
IMMUNOASSAY
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
NUCLEI
NUCLEOSIDES
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PURINES
RADIOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
RADIOISOTOPES
RIBOSIDES
SEPARATION PROCESSES
SYNTHESIS
TRACER TECHNIQUES
550601* - Medicine- Unsealed Radionuclides in Diagnostics