Further evidence implicating prostaglandin E sub 2 in the genesis of pyrogen fever

Journal Article · Tue Mar 01 00:00:00 EST 1988 · American Journal of Physiology; (USA)

OSTI ID:6808820

Coceani, F; Lees, J; Bishai, I ^[1]

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1. Hospital for Sick Children, Toronto, Ontario (Canada)

Conscious cats were used to study the effects of endotoxin and interleukin 1 (IL 1) on levels of prostaglandin (PG) E{sub 2} and thromboxane (TX) B{sub 2} (the stable TXA{sub 2} byproduct) in cerebrospinal fluid (CSF) from the third ventricle. Pyrogens were given intravenously or intraventricularly and prostanoids were measured by radioimmunoassay. PGE{sub 2} was normally less abundant than TXB{sub 2}, and its level increased severalfold during the sustained fever following intravenous endotoxin (bolus) or IL 1 (bolus plus infusion). PGE{sub 2} elevation preceded the fever and was maintained thereafter. Likewise, intraventricular pyrogens promoted PGE{sub 2} formation, and their effect was also manifest during the latent period of the fever. The PGE{sub 2} metabolite, 13,14-dihydro-15-keto-PGE{sub 2}, was not measurable in CSF from either afebrile or febrile animals. Basal content of PGE{sub 2}, on the other hand, was higher in animals pretreated with probenecid, confirming the importance of transport processes in removing prostanoids from brain. Unlike PGE{sub 2}, TXB{sub 2} levels did not change during the fever to intravenous endotoxin. TXB{sub 2} rose instead in response to intraventricular endotoxin, although the elevation did not extend beyond fever uprise. Furthermore, a TXA{sub 2} analog had inconsistent effects on body temperature, while a TXA{sub 2} antagonist did not interfere with endotoxin fever. These findings strongly support a causative role for PGE{sub 2} in the onset and progression of pyrogen fever. No evidence of a similar role was obtained for TXA{sub 2}.

• OSTI ID: 6808820
• Journal Information: American Journal of Physiology; (USA), Vol. 254:3; ISSN 0002-9513
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
FEVER
PATHOGENESIS
PROSTAGLANDINS
RADIOIMMUNOASSAY
SERINE PROTEINASES
BIOLOGICAL EFFECTS
CATS
CEREBROSPINAL FLUID
ENDOTOXINS
IODINE 125
LYMPHOKINES
PYROGENS
ANIMALS
ANTIGENS
BETA DECAY RADIOISOTOPES
BIOASSAY
BIOLOGICAL MATERIALS
BODY FLUIDS
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
GROWTH FACTORS
HYDROLASES
IMMUNOASSAY
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MATERIALS
MITOGENS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HYDROLASES
PROTEINS
RADIOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
RADIOISOTOPES
SYMPTOMS
TOXIC MATERIALS
TOXINS
TRACER TECHNIQUES
VERTEBRATES
551001* - Physiological Systems- Tracer Techniques