Assembly of RNA polymerase II preinitiation complexes before assembly of nucleosomes allows efficient initiation of transcription on nucleosomal templates
The authors have previously shown that assembly of nucleosomes on the DNA template blocks transcription initiation by RNA polymerase II in vitro. In the studies reported here, they demonstrate that assembly of a complete RNA polymerase II preinitiation complex before nucleosome assembly results in nucleosomal templates which support initiation in vitro as efficiently as naked DNA. Control experiments prove that the observations are not the result of slow displacemnt of nucleosomes by the transcription machinery during chromatin assembly, nor are they an artifact of inefficient nucleosome deposition on templates already bearing an RNA polymerase. Thus, the RNA polymerase II preinitiation complex appears to be resistant to disruption by subsequent nucleosome assembly.
- Research Organization:
- Lab. of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD (US)
- OSTI ID:
- 6805508
- Journal Information:
- Mol. Cell. Biol.; (United States), Vol. 8:8
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
NUCLEOSOMES
RNA POLYMERASES
BIOLOGICAL FUNCTIONS
TRANSCRIPTION
MOLECULAR BIOLOGY
BIOLOGICAL PATHWAYS
CHROMATIN
DNA SEQUENCING
EXPERIMENTAL DATA
IN VITRO
DATA
ENZYMES
FUNCTIONS
INFORMATION
NUCLEOTIDYLTRANSFERASES
NUMERICAL DATA
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES
550200* - Biochemistry