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Title: Cloning, sequencing, and expression of the gene coding for the human platelet. cap alpha. /sub 2/-adrenergic receptor

The gene for the human platelet ..cap alpha../sub 2/-adrenergic receptor has been cloned with oligonucleotides corresponding to the partial amino acid sequence of the purified receptor. The identity of this gene has been confirmed by the binding of ..cap alpha../sub 2/-adrenergic ligands to the cloned receptor expressed in Xenopus laevis oocytes. The deduced amino acid sequence is most similar to the recently cloned human ..beta../sub 2/- and ..beta../sub 1/-adrenergic receptors; however, similarities to the muscarinic cholinergic receptors are also evident. Two related genes have been identified by low stringency Southern blot analysis. These genes may represent additional ..cap alpha../sub 2/-adrenergic receptor subtypes.
Authors:
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Publication Date:
OSTI Identifier:
6805416
Resource Type:
Journal Article
Resource Relation:
Journal Name: Science (Washington, D.C.); (United States); Journal Volume: 238
Research Org:
Duke Univ. Medical Center, Durham, NC (USA)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; GENES; CLONING; DNA SEQUENCING; RECEPTORS; AMINO ACID SEQUENCE; SYMPATHOMIMETICS; GENE REGULATION; BLOOD PLATELETS; FROGS; LIGANDS; MAN; OOCYTES; AMPHIBIANS; ANIMALS; AQUATIC ORGANISMS; AUTONOMIC NERVOUS SYSTEM AGENTS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; DRUGS; GERM CELLS; MAMMALS; MATERIALS; MEMBRANE PROTEINS; MOLECULAR STRUCTURE; ORGANIC COMPOUNDS; PRIMATES; PROTEINS; STRUCTURAL CHEMICAL ANALYSIS; VERTEBRATES 550200* -- Biochemistry