Hydroxylation, conjugation and sulfation of bile acids in primary monolayer cultures of rat hepatocytes
Hydroxylation of lithocholic, chenodeoxycholic, deoxycholic and cholic acids was studied in monolayers of rat hepatocytes cultured for 76 h. The majority of added lithocholic and chenodeoxycholic acids was metabolized to beta-muricholic acid (56-76%). A small part of these bile acids (9%), however, and a considerable amount of deoxycholic and cholic acids (21%) were converted into metabolites more polar than cholic acid in the first culture period. Formation of these compounds decreased during the last day of culture. Bile acids synthesized after addition of (4-/sup 14/C)-cholesterol were almost entirely (97%) sulfated and/or conjugated, predominantly with taurine (54-66%), during culture. Sulfated bile acids were mainly composed of free bile acids. The ability of hepatocytes to sulfurylate bile acids declined with culture age. Thus, rat hepatocytes in primary monolayer culture are capable to sulfurylate bile acids and to hydroxylate trihydroxylated bile acids, suggesting formation of polyhydroxylated metabolites.
- Research Organization:
- Gaubius Institute TNO, Leiden (Netherlands)
- OSTI ID:
- 6804620
- Journal Information:
- Biochem. Biophys. Res. Commun.; (United States), Vol. 154:3
- Country of Publication:
- United States
- Language:
- English
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LIVER CELLS
METABOLITES
RATS
ANIMAL CELLS
ANIMALS
CARBOXYLIC ACIDS
HYDROXY COMPOUNDS
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MAMMALS
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550501* - Metabolism- Tracer Techniques