Carbon-14-ochratoxin A distribution in the Japanese quail (Coturnix coturnix japonica) monitored by whole body autoradiography

Journal Article · Sun May 01 00:00:00 EDT 1988 · Poult. Sci.; (United States)

DOI:https://doi.org/10.3382/ps.0670707· OSTI ID:6804566

Fuchs, R; Appelgren, L E; Hagelberg, S; Hult, K

Tissue distribution of the nephrotoxic mycotoxin ochratoxin A was characterized in laying Japanese quail by whole body autoradiography and scintillation counting using /sup 14/C-labelled toxin. Periodically for 8 days after one intravenous injection of 14 microCi/bird, corresponding to 70 ng/g body weight, birds were killed, frozen, and sagittal sections of the whole body were placed on X-ray film. In general, the ochratoxin disappeared from the avian body rapidly. Specific retention of radioactivity was seen as a ring-like distribution in yolks and growing follicles. After sectioning, organs and intestinal contents were removed from carcasses in a frozen condition, homogenized, extracted, chromatographed, and the radioactivity in fractions was measured by scintillation spectroscopy. High concentrations of ochratoxin A were found in gastric intestinal contents, probably originating from toxin excreted in the bile.

• Research Organization: Univ. of Zagreb (Yugoslavia)
• OSTI ID: 6804566
• Journal Information: Poult. Sci.; (United States), Vol. 67:5
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
TOXINS
TISSUE DISTRIBUTION
AUTORADIOGRAPHY
BIRDS
CARBON 14 COMPOUNDS
CHROMATOGRAPHY
INTRAVENOUS INJECTION
METABOLISM
RETENTION
SCINTILLATION COUNTING
WHOLE-BODY COUNTING
ANIMALS
ANTIGENS
COUNTING TECHNIQUES
DISTRIBUTION
INJECTION
INTAKE
LABELLED COMPOUNDS
MATERIALS
SEPARATION PROCESSES
TOXIC MATERIALS
VERTEBRATES
550501* - Metabolism- Tracer Techniques