Isolation and functional aspects of free luteal cells
Methods of luteal cell isolation employ enzymatic treatment of luteal tissue with collagenase and deoxyribonuclease. Additional enzymes such as hyaluronidase or Pronase are also used in some instances. Isolated luteal cells retain the morphological characteristics of steroid secreting cells after isolation. They contain mitochondria, variable amounts of lipid droplets, and an extensive smooth endoplasmic reticulum. Isolated luteal cells have been used in numerous studies to examine the regulation of steriodogenesis by luteinizing hormone (LH). LH receptor binding studies were employed to quantitate specific properties of hormone-receptor interaction in relation to cellular function. Binding of (/sup 125/I)LH to bovine luteal cells and membranes was compared and it was concluded that the enzymatic treatment used to isolate cells did not change the LH receptor binding kinetics.
- OSTI ID:
- 6801657
- Journal Information:
- Methods Enzymol.; (United States), Vol. 109
- Country of Publication:
- United States
- Language:
- English
Similar Records
Regulation versus modulation in GnRH receptor function
Subcellular distribution of ( sup 3 H)-dexamethasone mesylate binding sites in Leydig cells using electron microscope radioautography
Related Subjects
ANIMAL CELLS
MORPHOLOGY
ESTROUS CYCLE
LH
RADIORECEPTOR ASSAY
BIOCHEMICAL REACTION KINETICS
DNA-ASE
ENDOPLASMIC RETICULUM
EXPERIMENTAL DATA
HYALURONIDASE
IODINE 125
LABELLED COMPOUNDS
LIPIDS
MITOCHONDRIA
OVARIES
RECEPTORS
STEROIDS
TRACER TECHNIQUES
BETA DECAY RADIOISOTOPES
BODY
CELL CONSTITUENTS
DATA
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
ESTERASES
FEMALE GENITALS
GLYCOSYL HYDROLASES
GONADOTROPINS
GONADS
HORMONES
HYDROLASES
INFORMATION
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MEMBRANE PROTEINS
NUCLEI
NUMERICAL DATA
O-GLYCOSYL HYDROLASES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
PEPTIDE HORMONES
PHOSPHODIESTERASES
PITUITARY HORMONES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
550201* - Biochemistry- Tracer Techniques