Sodium azide-induced DNA single-strand breaks and DNA-protein crosslinks in barley embryos

Conference · Fri Jan 01 00:00:00 EST 1982

OSTI ID:6793450

Veleminsky, J; Kleinhofs, A; Nilan, R A

Barley (Hordeum vulgare L., cv. Himalaya) embryos germinated for two days in sterile culture were exposed to 5 mM and 10 mM NaN/sub 3/ in pH 3 buffer for 2 h. (/sup 3/H) DNA from the isolated nuclei was analyzed by alkaline elution from PVC filters. DNA single-strand breaks and DNA-protein crosslinks, detected by proteinase K digestion, were detected both immediately and 24 h after the azide treatment. Repair of these lesions during 24 h of posttreatment incubation of embryos in nutrient medium was not observed.

• Research Organization: Washington State Univ., Pullman (USA); Ceskoslovenska Akademie Ved, Prague. Ustav Experimentalni Botaniky
• DOE Contract Number: AT06-76EV72002
• OSTI ID: 6793450
• Report Number(s): DOE/EV/72002-63; CONF-820909-15; ON: DE83004908
• Resource Relation: Conference: National meeting of the American Chemical Society, Kansas City, MO, USA, 1 Sep 1982
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AZIDES
BIOLOGICAL EFFECTS
DNA
CROSS-LINKING
STRAND BREAKS
BARLEY
BIOLOGICAL REPAIR
EMBRYOS
EXPERIMENTAL DATA
LABELLED COMPOUNDS
SODIUM COMPOUNDS
TRITIUM
ALKALI METAL COMPOUNDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL RECOVERY
CEREALS
CHEMICAL REACTIONS
DATA
GRAMINEAE
GRASS
HYDROGEN ISOTOPES
INFORMATION
ISOTOPES
LIGHT NUCLEI
NITROGEN COMPOUNDS
NUCLEI
NUCLEIC ACIDS
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PLANTS
POLYMERIZATION
RADIOISOTOPES
RECOVERY
REPAIR
YEARS LIVING RADIOISOTOPES
560303* - Chemicals Metabolism & Toxicology- Plants- (-1987)