Differentiation-associated decrease in muscarinic receptor sensitivity in human neuroblastoma cells

Journal Article · Thu Jan 01 00:00:00 EST 1987 · J. Cell. Physiol.; (United States)

DOI:https://doi.org/10.1002/jcp.1041300122· OSTI ID:6792811

Heikkilae, J E; Scott, J G; Suominen, L A; Akerman, K E.O.

Muscarinic receptor-linked increases in intracellular free Ca/sup 2 +/ as measured with quin-2 and Ca/sup 2 +/ release from monolayers of cells have been measured in the human neuroblastoma cell line SH-SY5Y. Induction of differentiation with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) leads to a decrease in the sensitivity of the cells to low concentrations of agonists with respect to the induced increase in cytosolic free Ca/sup 2 +/ and stimulation of Ca/sup 2 +/ efflux. No decrease in agonist binding affinity was observed when the displacement of a labelled antagonist, /sup 3/H-NMS, by a non-labelled agonist was studied.

• Research Organization: Abo Academy, Finland
• OSTI ID: 6792811
• Journal Information: J. Cell. Physiol.; (United States), Vol. 130:1
• Country of Publication: United States
• Language: English

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