Identification of a UV-induced trans-acting protein that stimulates polyomavirus DNA replication

Journal Article · Tue Mar 01 00:00:00 EST 1988 · Journal of Virology; (USA)

OSTI ID:6790660

Ronai, Z A; Weinstein, I B ^[1]

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1. Columbia Univ., New York, NY (USA)

Previous studies provided indirect evidence that the ability of a variety of DNA-damaging agents to induce asynchronous polyomavirus DNA replication in the H3 rat fibroblast cell line is mediated by a trans-acting factor. Using an erythrocyte insertion technique to introduce protein fractions from UV-irradiated cells into unirradiated H3 cells, we have now obtained evidence that this factor is a 60-kilodalton protein. These findings provide evidence that DNA damage in mammalian cells induces a factor that can alter the replication of a viral DNA.

• OSTI ID: 6790660
• Journal Information: Journal of Virology; (USA), Vol. 62:3; ISSN 0022-538X
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
FIBROBLASTS
BIOLOGICAL RADIATION EFFECTS
POLYOMA VIRUS
DNA REPLICATION
PROTEINS
RADIOINDUCTION
DOSE-RESPONSE RELATIONSHIPS
PHOSPHORUS 32
RATS
ULTRAVIOLET RADIATION
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ELECTROMAGNETIC RADIATION
ISOTOPES
LIGHT NUCLEI
MAMMALS
MICROORGANISMS
NUCLEI
NUCLEIC ACID REPLICATION
ODD-ODD NUCLEI
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS ISOTOPES
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RODENTS
SOMATIC CELLS
VERTEBRATES
VIRUSES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture