Two types of T helper cells in mice: Differences in cellular immune functions and cytokine secretion - selective reduction of one type after total lymphoid irradiation
As observed from a large panel of mouse T helper clones, there are at least two subsets of CD4{sup +} T cells that both differ in function and demonstrate distinct patterns of cytokine secretion after antigen or mitogen stimulation. Th1 cells synthesize IL-2, INF-{gamma} and lymphotoxin. They produce a DTH reaction in the footpads of naive mice. In addition, Th1 cells are required for the generation of CTL, and they appear to augment IgG2a antibody production. In contrast, by secreting IL-4, IL-5, and IL-6, Th2 cells play an essential role in humoral immunity. TLI consists of high dose, fractionated irradiation delivered selectively to the major lymphoid tissues. Four to six weeks after TLI, the CD4{sup +} cells of the treated mice (counted as a percentage of the total spleen lymphocytes) recover to the similar levels as those in normal BALB/c mice. These CD4{sup +} cells can help normal syngeneic B cells to produce a vigorous antibody response to TNP-KLH in adoptive cell transfer experiments, but the same cells are inactive in the MLR, and they fail to transfer DTH in TNP-KLH primed syngeneic BALB/c mice.
- Research Organization:
- Stanford Univ., CA (USA)
- OSTI ID:
- 6789720
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
LYMPHATIC SYSTEM
BIOLOGICAL RADIATION EFFECTS
LYMPHOCYTES
BIOLOGICAL FUNCTIONS
LYMPHOKINES
SECRETION
BIOLOGICAL PATHWAYS
FRACTIONATED IRRADIATION
MICE
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
FUNCTIONS
GROWTH FACTORS
IRRADIATION
LEUKOCYTES
MAMMALS
MATERIALS
MITOGENS
ORGANIC COMPOUNDS
PROTEINS
RADIATION EFFECTS
RODENTS
SOMATIC CELLS
VERTEBRATES
560152* - Radiation Effects on Animals- Animals