Rapid isolation of intact, viable fetal cartilage models

Journal Article · Thu Apr 01 00:00:00 EST 1982 · Clin. Orthop.; (United States)

DOI:https://doi.org/10.1097/00003086-198204000-00051· OSTI ID:6778792

Schmidt, R R; Chepenik, K P; Paynton, B V; Cotler, J M

A rapid procedure is described for the isolation of viable, intact, femoral cartilage models (humeri and femora) obtained from pregnant rats on the 18th day of gestation. Viability of these models is demonstrated in an in vitro system where the incorporation of /sup 35/S-sulfate was linear with time of incubation and with numbers of cartilage models utilized. Treatment of cartilage models with ice-cold trichloroacetic acid and a boiling water bath prior to incubation with radiolabel, reduced the amount of radioactivity incorporated to 1.3% of that observed for models incubated by routine procedures. Furthermore, digestion of cartilage model homogenates with protease yielded a supernatant from which 51% to 57% of the radioactivity was precipitated as GAG. This method may also be used to isolate fetal cartilage models as early as the 16th day of gestation. with this system, specific biochemical parameters of mammalian fetal chondrogenesis may be surveyed in normally and abnormally developing fetal cartilage free of surrounding soft tissue.

• Research Organization: Department of Anatomy, Jefferson Medical College of Thomas Jefferson University, Philadelphia, PA
• OSTI ID: 6778792
• Journal Information: Clin. Orthop.; (United States), Vol. 164
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CARTILAGE
BIOLOGICAL MODELS
SULFUR 35
ISOTOPE APPLICATIONS
BIOLOGICAL LOCALIZATION
BONE JOINTS
CELL CULTURES
COLLAGEN
COMPARATIVE EVALUATIONS
FETUSES
PHYSIOLOGY
PREGNANCY
RATS
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CONNECTIVE TISSUE
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
ISOTOPES
LIGHT NUCLEI
MAMMALS
NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOISOTOPES
RODENTS
SCLEROPROTEINS
SKELETON
SULFUR ISOTOPES
TISSUES
VERTEBRATES
551001* - Physiological Systems- Tracer Techniques
550201 - Biochemistry- Tracer Techniques