Differences in susceptibility among mouse strains to infection with Plasmodium berghei (ANKA clone) sporozoites and its relationship to protection by gamma-irradiated sporozoites
- Walter Reed Army Institute of Research, Washington, DC (USA)
Three inbred mouse strains, C57BL/6 (H-2b), A/J (H-2a), and BALB/c (H-2d), and 1 outbred strain, CD-1, demonstrated differences in susceptibility to iv challenge with the ANKA clone of Plasmodium berghei. Mice were challenged with 100, 1,000, or 10,000 sporozoites, then evaluated daily beginning on day 4 for patency. CD-1 mice were further evaluated at challenge doses of 12,500, 25,000, and 50,000 sporozoites. C57BL/6 mice were the easiest to infect, with 90% becoming infected with 100 sporozoites. The outbred strain CD-1 was the most difficult to infect, requiring a challenge dose of 25,000 sporozoites/mouse in order to achieve a 100% infection rate. Mouse strains also demonstrated differences in their ability to be protected by intravenous immunization with gamma-irradiated sporozoites. A/J mice needed a minimum of 3 doses of irradiated sporozoites for protection against a challenge with 10,000 sporozoites. In contrast, BALB/c mice immunized with a single dose of 1,000 irradiated sporozoites are protected against a 10,000 sporozoite challenge. These data suggest that both infectivity and protection are genetically restricted and that susceptibility to infection may be inversely related to protection.
- OSTI ID:
- 6772766
- Journal Information:
- American Journal of Tropical Medicine and Hygiene; (USA), Vol. 42:4; ISSN 0002-9637
- Country of Publication:
- United States
- Language:
- English
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IMMUNITY
RADIOINDUCTION
MALARIA
VACCINES
PLASMODIUM
INFECTIVITY
BIOLOGICAL RADIATION EFFECTS
GAMMA RADIATION
GENETIC VARIABILITY
MICE
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL VARIABILITY
DISEASES
ELECTROMAGNETIC RADIATION
INFECTIOUS DISEASES
INVERTEBRATES
IONIZING RADIATIONS
MAMMALS
MICROORGANISMS
PARASITES
PARASITIC DISEASES
PROTOZOA
RADIATION EFFECTS
RADIATIONS
RODENTS
SPOROZOA
VERTEBRATES
553003* - Agriculture & Food Technology- Pest & Disease Control- (1987-)