Fed-batch approach to production of 2,3-butanediol by Klebsiella pneumoniae grown on high substrate concentrations

Journal Article · Thu Sep 01 00:00:00 EDT 1983 · Appl. Environ. Microbiol.; (United States)

OSTI ID:6771926

Yu, E K.C.; Saddler, J N

The bioconversion of sugars present in wood hemicellulose to 2,3-butanediol by Klebsiella pneumoniae grown on high sugar concentrations was investigated. When K. pneumoniae was grown under finite air conditions in the presence of added acetic acid, 50 g of D-glucose and D-xylose per liter could be converted to 25 and 27 g of butanediol per liter, respectively. The efficiency of bioconversion decreased with increasing sugar substrate concentrations (up to 200 g/liter). Butanediol production at low sugar substrate concentrations was less efficient when the organism was grown under aerobic conditions; however, final butanediol values were higher for cultures grown on an initial sugar concentration of 150 g/liter, particularly when the inoculum was first acclimatized to high sugar levels. When a double fed-batch approach (daily additions of sugars together with yeast extract) was used under aerobic conditions, up to 88 and 113 g of combined butanediol and acetyl methyl carbinol per liter could be obtained from the utilization of 190 g of D-xylose and 226 g of D-glucose per liter, respectively. 22 references.

• Research Organization: Forintek Canada Corp, Ottawa, Ontario
• OSTI ID: 6771926
• Journal Information: Appl. Environ. Microbiol.; (United States), Vol. 46:3
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
59 BASIC BIOLOGICAL SCIENCES
BUTANEDIOLS
PRODUCTION
KLEBSIELLA
SEMIBATCH CULTURE
AEROBIC CONDITIONS
BIOCONVERSION
CULTURE MEDIA
HEMICELLULOSE
OPTIMIZATION
SUBSTRATES
WOOD
ALCOHOLS
BACTERIA
CARBOHYDRATES
GLYCOLS
HYDROXY COMPOUNDS
MICROORGANISMS
ORGANIC COMPOUNDS
POLYSACCHARIDES
SACCHARIDES
140504* - Solar Energy Conversion- Biomass Production & Conversion- (-1989)
550700 - Microbiology