Genetics of bacteria that utilize one carbon compounds: Annual report, March 1, 1988--December 24, 1988
Bacteria that grow on methane, methanol and methylamine are a morphologically and physiologically diverse group of bacteria. Several of these bacteria have been shown to be clustered in two phylogenetically related groups by analysis of 16S RNA sequences. This information is useful for the synthesis of probes to detect methanotrophs in samples from bioreactors and in a variety of environments. We have observed considerable sequence homology in the methanol dehydrogenase genes of all gram negative methylotrophs. However, the upstream regulatory sequences of the two methylotrophic bacteria from which MDH genes have been completely sequenced have little similarity. The genetic maps of 12 genes required for MDH synthesis show several differences in two closely related methylotrophs. The MDH messenger RNA has a chemical half life of approximately one-minute although the protein represents 20% of the cellular protein. We now intend to map and sequence cloned genes encoding five proteins of Methylosinus trichosporium OB3B required for methane monooxygenase activity. We will continue to characterize the fourteen genes (and their gene products) required for MDH synthesis and will continue 16S rRNA sequencing in order to complete the molecular phylogeny of methanotrophic bacteria. 3 refs.
- Research Organization:
- Minnesota Univ., Navarre (USA). Freshwater Biological Inst.
- DOE Contract Number:
- FG02-88ER13862
- OSTI ID:
- 6771681
- Report Number(s):
- DOE/ER/13862-1; ON: DE89006058
- Resource Relation:
- Other Information: Portions of this document are illegible in microfiche products
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
METHANE
CATABOLISM
METHANOTROPHIC BACTERIA
GENETIC VARIABILITY
OXIDOREDUCTASES
DNA-CLONING
BIOLOGICAL PATHWAYS
COSMIDS
DNA SEQUENCING
GENETIC MAPPING
KREBS CYCLE
METHANOL
MOLECULAR BIOLOGY
PLASMIDS
PROGRESS REPORT
ALCOHOLS
ALKANES
BACTERIA
BIOLOGICAL VARIABILITY
CELL CONSTITUENTS
CLONING
DNA HYBRIDIZATION
DOCUMENT TYPES
ENZYMES
HYBRIDIZATION
HYDROCARBONS
HYDROXY COMPOUNDS
MAPPING
METABOLISM
MICROORGANISMS
ORGANIC COMPOUNDS
STRUCTURAL CHEMICAL ANALYSIS
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