De novo synthesis of adenine nucleotides in different skeletal muscle fiber types

Journal Article · Thu Sep 01 00:00:00 EDT 1988 · Am. J. Physiol.; (United States)

OSTI ID:6758048

Tullson, P C; John-Alder, H B; Hood, D A; Terjung, R L

Management of adenine nucleotide catabolism differs among skeletal muscle fiber types. This study evaluated whether there are corresponding differences in the rates of de novo synthesis of adenine nucleotide among fiber type sections of skeletal muscle using an isolated perfused rat hindquarter preparation. Label incorporation into adenine nucleotides from the (1-14C)glycine precursor was determined and used to calculate synthesis rates based on the intracellular glycine specific radioactivity. Results show that intracellular glycine is closely related to the direct precursor pool. Rates of de novo synthesis were highest in fast-twitch red muscle (57.0 +/- 4.0, 58.2 +/- 4.4 nmol.h-1.g-1; deep red gastrocnemius and vastus lateralis), relatively high in slow-twitch red muscle (47.0 +/- 3.1; soleus), and low in fast-twitch white muscle (26.1 +/- 2.0 and 21.6 +/- 2.3; superficial white gastrocnemius and vastus lateralis). Rates for four mixed muscles were intermediate, ranging between 32.3 and 37.3. Specific de novo synthesis rates exhibited a strong correlation (r = 0.986) with muscle section citrate synthase activity. Turnover rates (de novo synthesis rate/adenine nucleotide pool size) were highest in high oxidative muscle (0.82-1.06%/h), lowest in low oxidative muscle (0.30-0.35%/h), and intermediate in mixed muscle (0.44-0.55%/h). Our results demonstrate that differences in adenine nucleotide management among fiber types extends to the process of de novo adenine nucleotide synthesis.

• Research Organization: State Univ. of New York Health Science Center, Syracuse (USA)
• OSTI ID: 6758048
• Journal Information: Am. J. Physiol.; (United States), Vol. 255
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
MUSCLES
CATABOLISM
NUCLEOTIDES
ADENINES
CARBON 14 COMPOUNDS
GLYCINE
PERFUSED TISSUES
RATS
TRACER TECHNIQUES
AMINES
AMINO ACIDS
ANIMAL TISSUES
ANIMALS
ANTIMETABOLITES
AROMATICS
AZAARENES
BODY
CARBOXYLIC ACIDS
DRUGS
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
METABOLISM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PURINES
RODENTS
TISSUES
VERTEBRATES
550501* - Metabolism- Tracer Techniques