Stable isotope methodology in the pharmacokinetic studies of androgenic steroids in humans

Journal Article · Sun Apr 01 00:00:00 EST 1990 · Steroids; (USA)

OSTI ID:6749554

Shinohara, Y; Baba, S ^[1]

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1. Tokyo College of Pharmacy (Japan)

The use of stable isotopically labeled steroids combined with gas chromatography/mass spectrometry (GC/MS) has found a broad application in pharmacologic studies. Initially, stable isotopically labeled steroids served as the ideal analytic internal standard for GC/MS analysis; however, their in vivo use has expanded and has proven to be a powerful pharmacokinetic tool. We have successfully used stable isotope methodology to study the pharmacokinetic/bioavailability of androgens. The primary advantage of the technique is that endogenous and exogenous steroids with the same basic structure can be differentiated by using stable isotopically labeled analogs. The method was used to examine the pharmacokinetics of testosterone and testosterone propionate, and to clarify the influence of endogenous testosterone. Another advantage of the isotope methods is that steroidal drugs can be administered concomitantly in two formulations (e.g., solution and solid dosage). A single set of blood samples serves to describe the time course of the formulations being compared. This stable isotope coadministration technique was used to estimate the relative bioavailability of 17 alpha-methyltestosterone. 35 references.

• OSTI ID: 6749554
• Journal Information: Steroids; (USA), Vol. 55:4; ISSN 0039-128X
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
TESTOSTERONE
LABELLING
BIOLOGICAL AVAILABILITY
BODY FLUIDS
DEUTERIUM
GAS CHROMATOGRAPHY
MAN
MASS SPECTROSCOPY
REVIEWS
TRACER TECHNIQUES
ANDROGENS
ANDROSTANES
ANIMALS
BIOLOGICAL MATERIALS
CHROMATOGRAPHY
DOCUMENT TYPES
HORMONES
HYDROGEN ISOTOPES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
KETONES
LIGHT NUCLEI
MAMMALS
MATERIALS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PRIMATES
SEPARATION PROCESSES
SPECTROSCOPY
STABLE ISOTOPES
STEROID HORMONES
STEROIDS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques