Neutrophil myeloperoxidase destruction by ultraviolet irradiation
The peroxidase activity of enriched leukocyte preparations on coverslips was determined cytochemically with a newly developed method. The techniques utilizes diaminobenzidine medium and cupric nitrate intensification and is suitable for analysis with light microscopy, SEM, and TEM. Blood specimens from control individuals were studied with and without in vitro UV irradiation and compared with those from psoriasis patients exposed therapeutically to various types of UV in phototherapy. All UV irradiated samples showed diminished neutrophil myeloperoxidase (MP) activity although that of the principal eosinophil peroxidase was unaffected. The SEMs supported the contention that decreased neutrophil MP activity might be related to UV induced degranulation. It is believed to be possible, eventually, to equate the observed MP degranulation effect after UV irradiation with diminished ability to fight bacterial infections.
- Research Organization:
- North Carolina Univ., Chapel Hill, NC (USA)
- OSTI ID:
- 6743471
- Report Number(s):
- PB-90-216011/XAB; TRN: 90-027997
- Resource Relation:
- Other Information: Pub. in Jnl. of Electron Microscopy, 138-139(Jun 1988)
- Country of Publication:
- United States
- Language:
- English
Similar Records
Inactivation of transferrin iron binding capacity by the neutrophil myeloperoxidase system
Bioactivation of myelotoxic xenobiotics by human neutrophil myeloperoxidase
Related Subjects
NEUTROPHILS
BIOLOGICAL RADIATION EFFECTS
PEROXIDASES
ENZYME ACTIVITY
SKIN DISEASES
RADIOTHERAPY
BLOOD
INHIBITION
SCANNING ELECTRON MICROSCOPY
ULTRAVIOLET RADIATION
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD CELLS
BODY FLUIDS
DISEASES
ELECTROMAGNETIC RADIATION
ELECTRON MICROSCOPY
ENZYMES
LEUKOCYTES
MATERIALS
MEDICINE
MICROSCOPY
NUCLEAR MEDICINE
OXIDOREDUCTASES
RADIATION EFFECTS
RADIATIONS
RADIOLOGY
THERAPY
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture