Interferon induced by UV-irradiated murine cytomegalovirus decreases type C virus expression in BALB/c cells treated by 5-iodo-deoxyuridine or cycloheximide

Journal Article · Sat Jan 01 00:00:00 EST 1983 · J. Interferon Res.; (United States)

DOI:https://doi.org/10.1089/jir.1983.3.451· OSTI ID:6743100

Sergiescu, D

UV-irradiated mouse cytomegalovirus (MCMV) activates at low incidence (2.0 X 10(-4) - 8.0 X 10(-4)) a xenotropic type C virus in Kirsten sarcoma virus-transformed nonproducer BALB 3T3 (KBALB) cells. When KBALB cells are treated with UV-MCMV and subsequently with potent chemical inducers, such as 5-Iododeoxyuridine (IdUrd) or cycloheximide, the activation frequencies of type C virus are significantly lower than in controls exposed to the chemical inducers alone: 60% for IdUrd and 44% for cycloheximide. This decrease of activation appears to be mediated by endogenous interferon (IFN) induced by UV-MCMV in KBALB cells, as could be proven by neutralization of the inhibitory activity. Indeed, after exposure of UV-MCMV-infected cells to anti-IFN gamma globulin, the level of C type virus increases, reaching values obtained with IdUrd or cycloheximide in the absence of UV-MCMV. The inhibitory activity associated with UV-MCMV is dose-dependent and the xenotropic type C virus appears to be more sensitive than the ecotropic (N-tropic) retrovirus of BALB/c cells.

• Research Organization: Institut National de la Sante et de la Recherche Medicale, Hopital Saint Vincent de Paul, Paris, France
• OSTI ID: 6743100
• Journal Information: J. Interferon Res.; (United States), Vol. 3:4
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
INTERFERON
BIOLOGICAL EFFECTS
VIRUSES
HOST-CELL REACTIVATION
CYCLOHEXIMIDE
INHIBITION
IODODEOXYURIDINE
ULTRAVIOLET RADIATION
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMETABOLITES
AZINES
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DRUGS
ELECTROMAGNETIC RADIATION
FUNGICIDES
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IODOURACILS
MICROORGANISMS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC IODINE COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASITES
PESTICIDES
PYRIMIDINES
RADIATIONS
RECOVERY
REPAIR
RIBOSIDES
URACILS
560131* - Radiation Effects on Microorganisms- Basic Studies- (-1987)