Clonidine promotes the accumulation of /sup 45/Ca in pancreatic beta-cell organelles

Journal Article · Thu Dec 01 00:00:00 EST 1983 · Res. Commun. Chem. Pathol. Pharmacol.; (United States)

OSTI ID:6742940

Andersson, T; Nygren, P

Glucose-stimulated insulin release from pancreatic islets of ob/ob-mice was inhibited by 10(-9) M of the alpha 2-adrenergic agonist clonidine. This inhibitory effect was abolished by 10(-7) M of the antagonist yohimbine. Loading the islets with /sup 45/Ca during the clonidine exposure followed by isolation of subcellular fractions under conditions known to minimize the /sup 45/Ca redistribution resulted in increased accumulation of the isotope in the mitochondrial and microsomal fractions. It is suggested that clonidine inhibits glucose-stimulated insulin release by increasing the organelle sequestration of Ca2+.

• Research Organization: Univ. of Uppsala, Sweden
• OSTI ID: 6742940
• Journal Information: Res. Commun. Chem. Pathol. Pharmacol.; (United States), Vol. 42:3
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CALCIUM 45
UPTAKE
DRUGS
BIOLOGICAL EFFECTS
ADRENALINE
INHIBITION
INSULIN
MICE
MICROSOMES
MITOCHONDRIA
PANCREAS
STIMULATION
ADRENAL HORMONES
ALKALINE EARTH ISOTOPES
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CALCIUM ISOTOPES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ENDOCRINE GLANDS
EVEN-ODD NUCLEI
GLANDS
HORMONES
INTERMEDIATE MASS NUCLEI
ISOTOPES
MAMMALS
NEUROREGULATORS
NUCLEI
ORGANOIDS
ORGANS
PEPTIDE HORMONES
RADIOISOTOPES
RODENTS
STEROID HORMONES
SYMPATHOMIMETICS
VERTEBRATES
560172* - Radiation Effects- Nuclide Kinetics & Toxicology- Animals- (-1987)