Effect of diphenylhydantoin and cortisol on the cell cycle

Journal Article · Wed Jun 01 00:00:00 EDT 1988 · Proc. Soc. Exp. Biol. Med.; (United States)

DOI:https://doi.org/10.3181/00379727-188-42724· OSTI ID:6736177

MacKinney, Jr, A A; Knobeloch, L; Norback, D

Normal human lymphocytes cultured in the presence of phytohemagglutinin were blocked in G/sub 0/G/sub 1/ when diphenylhydantoin (DPH) or cortisol 3.6 x 10/sup -4/ M was added at the beginning of culture. The suppression of culture growth was analyzed by flow cytometry and confirmed by (/sup 3/H)thymidine incorporation and mitotic rate analysis. The correlation of these measurements with flow cytometry was good for DNA synthesis and excellent for mitosis. There was an additive effect of the G/sub 0/G/sub 1/ retention of cells when both drugs were present in the culture. These data may partially explain the suppression of cell-mediated immunity which occurs in DPH-treated patients.

• Research Organization: William S. Middleton Memorial Veterans Hospital, Madison, WI (USA)
• OSTI ID: 6736177
• Journal Information: Proc. Soc. Exp. Biol. Med.; (United States), Vol. 188:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANTICONVULSANTS
BIOLOGICAL EFFECTS
HYDROCORTISONE
LYMPHOCYTES
CELL CYCLE
CELL CULTURES
CELL FLOW SYSTEMS
COMPARATIVE EVALUATIONS
IMMUNOSUPPRESSION
MITOTIC DELAY
PATIENTS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ADRENAL HORMONES
ANIMAL CELLS
AZINES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
CONNECTIVE TISSUE CELLS
CORTICOSTEROIDS
DRUGS
GLUCOCORTICOIDS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KETONES
LABELLED COMPOUNDS
LEUKOCYTES
MATERIALS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PREGNANES
PYRIMIDINES
RIBOSIDES
SOMATIC CELLS
STEROIDS
550301* - Cytology- Tracer Techniques