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Title: Autoradiographic localization of a gluten peptide during organ culture of human duodenal mucosa

An 125I-labeled subfraction of Frazer's fraction III (molecular weight, 8,000) was added to the culture medium during organ culture of duodenal biopsies from two patients with celiac disease in exacerbation. The isotope-labeled gluten peptide was localized by autoradiography after 6, 12, and 24 h of culture. At 6 h, labeling was located mainly in the basal layers of the biopsies. The tissue was well preserved. After 12 h in culture, the labeling had spread to the lamina propria and the crypts. A few grains were located over enterocytes and desquamated cells. Moderate histological signs of toxicity were observed. After 24 h, there was marked toxic deterioration, comparable to that seen after culture with alpha-gliadin. Labeling had spread throughout the entire section. There seemed to be no specificity of the binding, for the entire section was affected. Culture with the identical gluten fraction, in the radionegative state, produced histological deterioration comparable to that seen after exposure to the isotope-labeled peptide. Gluten peptides are presented to the target cells in a unique way during organ culture, different from in vivo conditions. This may influence the results when the organ culture method is used to investigate the pathogenesis of celiac disease.
Authors:
;
Publication Date:
OSTI Identifier:
6723836
Resource Type:
Journal Article
Resource Relation:
Journal Name: J. Pediatr. Gastroenterol. Nutr.; (United States); Journal Volume: 2:3
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; PEPTIDES; AUTORADIOGRAPHY; SMALL INTESTINE; TISSUE CULTURES; BIOLOGICAL LOCALIZATION; DIGESTIVE SYSTEM; DISEASES; GLUTIN; IODINE 125; LABELLED COMPOUNDS; MUCOUS MEMBRANES; PATHOLOGY; PATIENTS; TRACER TECHNIQUES; BETA DECAY RADIOISOTOPES; BODY; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; GASTROINTESTINAL TRACT; INTERMEDIATE MASS NUCLEI; INTESTINES; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MEMBRANES; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANS; PROTEINS; RADIOISOTOPES; SCLEROPROTEINS 550901* -- Pathology-- Tracer Techniques