Effect of Vitreoscilla hemoglobin expression on growth and specific tissue plasminogen activator productivity in recombinant Chinese hamster ovary cells

Pendse, G J; Bailey, J E

doi:10.1002/bit.260441114

Title: Effect of Vitreoscilla hemoglobin expression on growth and specific tissue plasminogen activator productivity in recombinant Chinese hamster ovary cells

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Abstract

Previous studies suggest that secretion of cloned proteins synthesized by recombinant Chinese hamster ovary (CHO) cells can be adenosine triphosphate (ATP) limited. Other research indicates that the presence of cloned Vitreoscilla hemoglobin (VHb) enhances ATP production in oxygen-limited Escherichia coli. To evaluate the influence of VHb expression on recombinant CHO cell productivity, the vhb gene has been fused to the mouse mammary tumor virus (MMTV) promoter and cloned in a CHO cell line previously engineered to express human tissue plasminogen activator (tPA). Western blot analysis confirms dexamethasone-inducible VHb expression in all of the clones tested. Batch cultivation experiments with one VHb-expressing clone and the parental CHO-tPA cells show a reduced specific growth rate in the VHb-expressing cells. The VHb-expressing clone exhibits specific tPA production 40 to 100% greater than the parental CHO-tPA culture.

Authors:

Pendse, G J; Bailey, J E ^[1]

California Inst. of Tech., Pasadena, CA (United States). Dept. of Chemical Engineering

Publication Date:: Thu Dec 01 00:00:00 EST 1994

OSTI Identifier:: 6723195

Resource Type:: Journal Article

Journal Name:: Biotechnology and Bioengineering; (United States)

Additional Journal Information:: Journal Volume: 44:11; Journal ID: ISSN 0006-3592

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; CHO CELLS; GROWTH; HEMOGLOBIN; GENES; BIOTECHNOLOGY; CLONING; GENE REGULATION; POST-TRANSLATION MODIFICATION; RECOMBINANT DNA; ANIMAL CELLS; CARBOXYLIC ACIDS; DNA; GLOBINS; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; NUCLEIC ACIDS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PIGMENTS; PORPHYRINS; PROTEINS; 550200* - Biochemistry

Citation Formats


                    Pendse, G J, and Bailey, J E. Effect of Vitreoscilla hemoglobin expression on growth and specific tissue plasminogen activator productivity in recombinant Chinese hamster ovary cells.  United States: N. p., 1994. 
        Web.  doi:10.1002/bit.260441114.

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                    Pendse, G J, & Bailey, J E. Effect of Vitreoscilla hemoglobin expression on growth and specific tissue plasminogen activator productivity in recombinant Chinese hamster ovary cells.  United States.  https://doi.org/10.1002/bit.260441114

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                    Pendse, G J, and Bailey, J E. 1994.  
        "Effect of Vitreoscilla hemoglobin expression on growth and specific tissue plasminogen activator productivity in recombinant Chinese hamster ovary cells".  United States.  https://doi.org/10.1002/bit.260441114.

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@article{osti_6723195,

  title        = {Effect of Vitreoscilla hemoglobin expression on growth and specific tissue plasminogen activator productivity in recombinant Chinese hamster ovary cells},

  author       = {Pendse, G J and Bailey, J E},

  abstractNote = {Previous studies suggest that secretion of cloned proteins synthesized by recombinant Chinese hamster ovary (CHO) cells can be adenosine triphosphate (ATP) limited. Other research indicates that the presence of cloned Vitreoscilla hemoglobin (VHb) enhances ATP production in oxygen-limited Escherichia coli. To evaluate the influence of VHb expression on recombinant CHO cell productivity, the vhb gene has been fused to the mouse mammary tumor virus (MMTV) promoter and cloned in a CHO cell line previously engineered to express human tissue plasminogen activator (tPA). Western blot analysis confirms dexamethasone-inducible VHb expression in all of the clones tested. Batch cultivation experiments with one VHb-expressing clone and the parental CHO-tPA cells show a reduced specific growth rate in the VHb-expressing cells. The VHb-expressing clone exhibits specific tPA production 40 to 100% greater than the parental CHO-tPA culture.},

  doi          = {10.1002/bit.260441114},

  url          = {https://www.osti.gov/biblio/6723195},
  journal      = {Biotechnology and Bioengineering; (United States)},

  issn         = {0006-3592},

  number       = ,

  volume       = 44:11,

  place        = {United States},

  year         = {Thu Dec 01 00:00:00 EST 1994},

  month        = {Thu Dec 01 00:00:00 EST 1994}

}

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https://doi.org/10.1002/bit.260441114

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