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Title: Inhibition of Rev-mediated HIV-1 expression by an RNA binding protein encoded by the interferon-inducible 9-27 gene

Abstract

Interferon inhibits expression of human immunodeficiency virus type-1 (HIV-1) through unknown mechanisms. A gene inducible by interferon-[alpha] (IFN-[alpha]) and interferon-[gamma] (IFN-[gamma]) was isolated by screening of a human complementary DNA library for proteins binding to the Rev-responsive element (RRE) of HIV-1. The product of this gene, RBP9-27, was shown to bind RNA in vitro and to inhibit HIV-1 expression after transfection into human cells. RBP9-27 primarily inhibited Rev-dependent posttransscriptional steps of viral gene expression. Thus, RBP9-27 is a cellular factor that antagonizes Rev function. These results suggest an inteferon-induced antiviral mechanism operating through the induction of RNA binding proteins such as RBP9-27. Elucidation of RBP9-27 function may lead to a better understanding of the mechanism of interferon action during HIV-1 infection. 29 refs., 4 figs.

Authors:
; ; ; ; ;  [1]
  1. National Cancer Inst., Frederick, MD (United States)
Publication Date:
OSTI Identifier:
6717038
Resource Type:
Journal Article
Journal Name:
Science (Washington, D.C.); (United States)
Additional Journal Information:
Journal Volume: 259:5099; Journal ID: ISSN 0036-8075
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; AIDS VIRUS; INHIBITION; RNA PROCESSING; DNA; GENE REGULATION; GENES; IN VITRO; INDUCTION; INTERFERON; PROTEINS; RNA; SCREENING; GROWTH FACTORS; LYMPHOKINES; MICROORGANISMS; MITOGENS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; PARASITES; VIRUSES; 550400* - Genetics

Citation Formats

Constantoulakis, P, Campbell, M, Felber, B K, Nasioulas, G, Afonina, E, and Pavlakis, G N. Inhibition of Rev-mediated HIV-1 expression by an RNA binding protein encoded by the interferon-inducible 9-27 gene. United States: N. p., 1993. Web. doi:10.1126/science.7680491.
Constantoulakis, P, Campbell, M, Felber, B K, Nasioulas, G, Afonina, E, & Pavlakis, G N. Inhibition of Rev-mediated HIV-1 expression by an RNA binding protein encoded by the interferon-inducible 9-27 gene. United States. https://doi.org/10.1126/science.7680491
Constantoulakis, P, Campbell, M, Felber, B K, Nasioulas, G, Afonina, E, and Pavlakis, G N. 1993. "Inhibition of Rev-mediated HIV-1 expression by an RNA binding protein encoded by the interferon-inducible 9-27 gene". United States. https://doi.org/10.1126/science.7680491.
@article{osti_6717038,
title = {Inhibition of Rev-mediated HIV-1 expression by an RNA binding protein encoded by the interferon-inducible 9-27 gene},
author = {Constantoulakis, P and Campbell, M and Felber, B K and Nasioulas, G and Afonina, E and Pavlakis, G N},
abstractNote = {Interferon inhibits expression of human immunodeficiency virus type-1 (HIV-1) through unknown mechanisms. A gene inducible by interferon-[alpha] (IFN-[alpha]) and interferon-[gamma] (IFN-[gamma]) was isolated by screening of a human complementary DNA library for proteins binding to the Rev-responsive element (RRE) of HIV-1. The product of this gene, RBP9-27, was shown to bind RNA in vitro and to inhibit HIV-1 expression after transfection into human cells. RBP9-27 primarily inhibited Rev-dependent posttransscriptional steps of viral gene expression. Thus, RBP9-27 is a cellular factor that antagonizes Rev function. These results suggest an inteferon-induced antiviral mechanism operating through the induction of RNA binding proteins such as RBP9-27. Elucidation of RBP9-27 function may lead to a better understanding of the mechanism of interferon action during HIV-1 infection. 29 refs., 4 figs.},
doi = {10.1126/science.7680491},
url = {https://www.osti.gov/biblio/6717038}, journal = {Science (Washington, D.C.); (United States)},
issn = {0036-8075},
number = ,
volume = 259:5099,
place = {United States},
year = {Fri Feb 26 00:00:00 EST 1993},
month = {Fri Feb 26 00:00:00 EST 1993}
}