Relationship between lignin degradation and production of reduced oxygen species by Phanerochaete chrysosporium
The relationship between the production of reduced oxygen species, hydrogen peroxide (H/sub 2/O/sub 2/), superoxide (O/sub 2//sup -/), and hydroxyl radical (.OH), and the oxidation of synthetic lignin to CO/sub 2/ was studied in whole cultures of the white-rot fungus Phanerochaete chrysosporium Burds. The kinetics of the synthesis of H/sub 2/O/sub 2/ coincided with the appearance of the ligninolytic system; also, H/sub 2/O/sub 2/ production was markedly enhanced by growth under 100% O/sub 2/, mimicing the increase in ligninolytic activity characteristic of cultures grown under elevated oxygen tension. Lignin degradation by whole cultures was inhibited by a specific H/sub 2/O/sub 2/ scavenger, catalase, implying a role for H/sub 2/O/sub 2/ in the degradative process. Superoxide dismutase also inhibited lignin degradation, suggesting that O/sub 2//sup -/ is also involved in the breakdown of lignin. The production of .OH was assayed in whole cultures by a benzoate decarboxylation assay. Neither the kinetics of .OH synthesis nor the final activity of its producing system obtained under 100% O/sub 2/ correlated with that of the lignin-degrading system. However, lignin degradation was inhibited by compounds which react with .OH. It is concluded that H/sub 2/O/sub 2/, and perhaps O/sub 2//sup -/, are involved in lignin degradation; because these species are relatively unreactive per se, their role must be indirect. Conclusions about a role for .OH in ligninolysis could not be reached. (Refs. 28).
- Research Organization:
- Univ. of Wisconsin, Madison
- OSTI ID:
- 6711442
- Journal Information:
- Appl. Environ. Microbiol.; (United States), Vol. 46:5
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
HYDROGEN PEROXIDE
BIOSYNTHESIS
HYDROXYL RADICALS
LIGNIN
BIODEGRADATION
PHANEROCHAETE
BATCH CULTURE
SUPEROXIDE RADICALS
BIOCHEMICAL REACTION KINETICS
CATALASE
INHIBITION
OXIDATION
SUPEROXIDE DISMUTASE
CARBOHYDRATES
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
DECOMPOSITION
ENZYMES
FUNGI
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
KINETICS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PEROXIDASES
PEROXIDES
PLANTS
POLYSACCHARIDES
PORPHYRINS
RADICALS
REACTION KINETICS
SACCHARIDES
SYNTHESIS
140504* - Solar Energy Conversion- Biomass Production & Conversion- (-1989)
550700 - Microbiology