Effect of exercise and obesity on skeletal muscle amino acid uptake

Miscellaneous · Fri Jan 01 00:00:00 EST 1988

OSTI ID:6709687

Friedman, J E

To determine if amino acid uptake by muscle of the obese Zucker rat is impaired, epitrochlearis (EPI) and soleus strip (SOL) muscles from 32 pairs of female lean (Fa/-) and obese (fa/fa) Zucker rats were incubated using ({sup 14}C){alpha}-aminoisobutyric acid (AIB). Because contractile activity also influences amino acid uptake, the effect of acute endurance exercise on amino acid uptake by skeletal muscle from lean and obese rats was also studied. Muscle wet and dry weights were similar in lean and obese rats. However, both muscle protein content and concentration from obese rats were significantly reduced. In preliminary studies, pinning EPI at resting length during incubation significantly increased AIB uptake and reduced muscle water accumulation. AIB uptake was similar in stripped and intact SOL. Lean and obese rats were studied at rest or following a 1 hr treadmill run at 8% grade Muscles were pinned, and preincubated for 30 min at 37{degree}C in Krebs Ringer bicarbonate buffer (KRB) containing 5mM glucose under 95:5 O{sub 2}/CO{sub 2}, followed by 30, 60, 120, or 180 min of incubation in KRB with 0.5 mM AIB, ({sup 14}C)-AIB to measure amino acid, and ({sup 3}H)-inulin to determine extracellular water.

• Research Organization: Kent State Univ., OH (USA)
• OSTI ID: 6709687
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
UPTAKE
EXERCISE
BIOLOGICAL EFFECTS
ADIPOSE TISSUE
CARBON 14 COMPOUNDS
INULIN
MUSCLES
RATS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMAL TISSUES
ANIMALS
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
CONNECTIVE TISSUE
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
POLYSACCHARIDES
RODENTS
SACCHARIDES
TISSUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques