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Title: Distribution and effects of intravenous lead in the fetoplacental unit of the rat

Abstract

Lead metabolism was studied in the fetoplacental unit (FPU) of Wistar rats during the genesis of developmental abnormalities and embryonic death. Female rats were injected iv with tracer /sup 210/Pb(NO/sub 3/)/sub 2/, alone or in combination with 5 or 25 mg Pb(NO/sub 3/)/sub 2//kg, at 9 or 15 days of gestation (dg). The distribution of lead and its effects were determined in the FPUs during the ensuing 30-h period and at 20 dg. Hemorrhage of the egg cylinder was noted as early as 6 h postinjection of 25 mg/kg at 9 dg. By 20 dg, fetuses exhibited characteristic stunting and external malformations (gastrochisis and severe skeletal defects). Administration of this dose at 15 dg produced petechial hemorrhage in fetal brain within 90 min; more massive hemorrhage was a consistent observation by 24 h. At 20 dg, embryo mortality was 44% in rats injected with 25 mg/kg at 9 dg and 100% in those injected at 15 dg. At 90 min after injection, lead content of 15-dg FPUs was 10 times that of the 9-dg FPUs, but the weights of the 15-dg FPUs were 16 times greater. Values remained relatively constant in 15-dg FPUs for 30 h, but early clearance wasmore » observed after injection at 9 dg, with a return to 90-min values by 20 dg. In the 15-dg FPUs, placental clearance was followed by fetal lead incorporation, which reached a maximum at 6 h. Fetal lead values were constant from 6 to 30 h after injection at tracer and 5-mg/kg dose levels, but values increased progressively at 25 mg/kg. Both temporal and quantitative relationships of fetal lead metabolism were disrupted by the 25-mg/kg dose, but the nature of the effect was determined by the stage of fetal development at exposure.« less

Authors:
; ;
Publication Date:
Research Org.:
Pacific Northwest Lab., Richland, WA
OSTI Identifier:
6707121
DOE Contract Number:  
AC06-76RL01830
Resource Type:
Journal Article
Journal Name:
J. Toxicol. Environ. Health; (United States)
Additional Journal Information:
Journal Volume: 9
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; LEAD; BIOLOGICAL EFFECTS; PRENATAL EXPOSURE; LEAD 210; TISSUE DISTRIBUTION; BRAIN; CONGENITAL MALFORMATIONS; DOSE-RESPONSE RELATIONSHIPS; EXPERIMENTAL DATA; HEMORRHAGE; INTRAVENOUS INJECTION; LEAD NITRATES; LIVER; MEMBRANES; METABOLISM; MORTALITY; PLACENTA; RATS; TRACER TECHNIQUES; WEIGHT; ALPHA DECAY RADIOISOTOPES; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BODY; CENTRAL NERVOUS SYSTEM; DATA; DIGESTIVE SYSTEM; DISTRIBUTION; ELEMENTS; EVEN-EVEN NUCLEI; FETAL MEMBRANES; GLANDS; HEAVY NUCLEI; INFORMATION; INJECTION; INTAKE; ISOTOPE APPLICATIONS; ISOTOPES; LEAD COMPOUNDS; LEAD ISOTOPES; MALFORMATIONS; MAMMALS; METALS; NERVOUS SYSTEM; NITRATES; NITROGEN COMPOUNDS; NUCLEI; NUMERICAL DATA; ORGANS; OXYGEN COMPOUNDS; PATHOLOGICAL CHANGES; RADIOISOTOPES; RODENTS; SYMPTOMS; VERTEBRATES; YEARS LIVING RADIOISOTOPES; 560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987); 551001 - Physiological Systems- Tracer Techniques; 560172 - Radiation Effects- Nuclide Kinetics & Toxicology- Animals- (-1987)

Citation Formats

Hackett, P L, Hess, J O, and Sikov, M R. Distribution and effects of intravenous lead in the fetoplacental unit of the rat. United States: N. p., 1982. Web. doi:10.1080/15287398209530222.
Hackett, P L, Hess, J O, & Sikov, M R. Distribution and effects of intravenous lead in the fetoplacental unit of the rat. United States. https://doi.org/10.1080/15287398209530222
Hackett, P L, Hess, J O, and Sikov, M R. 1982. "Distribution and effects of intravenous lead in the fetoplacental unit of the rat". United States. https://doi.org/10.1080/15287398209530222.
@article{osti_6707121,
title = {Distribution and effects of intravenous lead in the fetoplacental unit of the rat},
author = {Hackett, P L and Hess, J O and Sikov, M R},
abstractNote = {Lead metabolism was studied in the fetoplacental unit (FPU) of Wistar rats during the genesis of developmental abnormalities and embryonic death. Female rats were injected iv with tracer /sup 210/Pb(NO/sub 3/)/sub 2/, alone or in combination with 5 or 25 mg Pb(NO/sub 3/)/sub 2//kg, at 9 or 15 days of gestation (dg). The distribution of lead and its effects were determined in the FPUs during the ensuing 30-h period and at 20 dg. Hemorrhage of the egg cylinder was noted as early as 6 h postinjection of 25 mg/kg at 9 dg. By 20 dg, fetuses exhibited characteristic stunting and external malformations (gastrochisis and severe skeletal defects). Administration of this dose at 15 dg produced petechial hemorrhage in fetal brain within 90 min; more massive hemorrhage was a consistent observation by 24 h. At 20 dg, embryo mortality was 44% in rats injected with 25 mg/kg at 9 dg and 100% in those injected at 15 dg. At 90 min after injection, lead content of 15-dg FPUs was 10 times that of the 9-dg FPUs, but the weights of the 15-dg FPUs were 16 times greater. Values remained relatively constant in 15-dg FPUs for 30 h, but early clearance was observed after injection at 9 dg, with a return to 90-min values by 20 dg. In the 15-dg FPUs, placental clearance was followed by fetal lead incorporation, which reached a maximum at 6 h. Fetal lead values were constant from 6 to 30 h after injection at tracer and 5-mg/kg dose levels, but values increased progressively at 25 mg/kg. Both temporal and quantitative relationships of fetal lead metabolism were disrupted by the 25-mg/kg dose, but the nature of the effect was determined by the stage of fetal development at exposure.},
doi = {10.1080/15287398209530222},
url = {https://www.osti.gov/biblio/6707121}, journal = {J. Toxicol. Environ. Health; (United States)},
number = ,
volume = 9,
place = {United States},
year = {Fri Jan 01 00:00:00 EST 1982},
month = {Fri Jan 01 00:00:00 EST 1982}
}