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Title: Enhancement of bone marrow allografts from nude mice into mismatched recipients by T cells void of graft-versus-host activity

Transplantation of 8 x 10(6) C57BL/6-Nu+/Nu+ (nude) bone marrow cells into C3H/HeJ recipients after conditioning with 8 Gy of total body irradiation has resulted in a markedly higher rate of graft rejection or graft failure compared to that found in recipients of normal C57BL/6 or C57BL/6-Bg+/Bg+ (beige) T-cell-depleted bone marrow. Mixing experiments using different numbers of nude bone marrow cells with or without mature thymocytes (unagglutinated by peanut agglutinin) revealed that engraftment of allogeneic T-cell-depleted bone marrow is T-cell dependent. To ensure engraftment, a large inoculum of nude bone marrow must be supplemented with a trace number of donor T cells, whereas a small bone marrow dose from nude donors requires a much larger number of T cells for engraftment. Marked enhancement of donor type chimerism was also found when F1 thymocytes were added to nude bone marrow cells, indicating that the enhancement of bone marrow engraftment by T cells is not only mediated by alloreactivity against residual host cells but may rather be generated by growth factors, the release of which may require specific interactions between T cells and stem cells or between T cells and bone marrow stroma cells.
Authors:
; ; ; ; ;  [1]
  1. (Weizmann Institute of Science, Rehovot (Israel))
Publication Date:
OSTI Identifier:
6703946
Resource Type:
Journal Article
Resource Relation:
Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA); Journal Volume: 87:12
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; BONE MARROW CELLS; TRANSPLANTS; LYMPHOCYTES; BIOLOGICAL FUNCTIONS; GRAFT-HOST REACTION; LECTINS; MICE; RADIATION CHIMERAS; STEM CELLS; SURVIVAL TIME; THYMOCYTES; WHOLE-BODY IRRADIATION; ANIMAL CELLS; ANIMALS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CHIMERAS; CONNECTIVE TISSUE CELLS; EXTERNAL IRRADIATION; FUNCTIONS; IRRADIATION; LEUKOCYTES; MAMMALS; MATERIALS; MOSAICISM; RODENTS; SOMATIC CELLS; VERTEBRATES 560152* -- Radiation Effects on Animals-- Animals