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Title: The cellular and molecular toxicity of lead in primary and clonal osteoblastic bone cells

Abstract

First, steady state kinetic models of lead metabolism and calcium homeostasis were developed in both primary and clonal osteoblastic bone cells. Secondly, the effect of lead on cellular calcium homeostasis was determined. Finally, the effect of lead on 1,25 (OH){sub 2}D{sub 3} induced production of osteocalcin, a protein synthesized and secreted by osteoblasts, was investigated. Lead metabolism in osteoblastic bone cells was characterized by three intracellular pools. The largest of these, S{sub 3}, included mitochondrial lead and accounted for 70 percent of total cell lead in primary osteoblastic bone cells and 85 percent of total lead in clonal osteoblastic bone cells. None of the kinetic pools were saturated at lead concentrations up to 100 {mu}M lead. Calcium homeostasis in osteoblastic bone cells was also described by a three compartment, intracellular kinetic model.

Authors:
Publication Date:
Research Org.:
Arkansas Univ., Fayetteville, AR (USA)
OSTI Identifier:
6703681
Resource Type:
Miscellaneous
Resource Relation:
Other Information: Thesis (Ph. D.)
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; CALCIUM; HOMEOSTASIS; LEAD; TOXICITY; PROTEINS; BIOSYNTHESIS; BONE CELLS; METABOLISM; MITOCHONDRIA; ALKALINE EARTH METALS; ANIMAL CELLS; CELL CONSTITUENTS; CONNECTIVE TISSUE CELLS; ELEMENTS; METALS; ORGANIC COMPOUNDS; ORGANOIDS; SOMATIC CELLS; SYNTHESIS; 560300* - Chemicals Metabolism & Toxicology

Citation Formats

Long, G J. The cellular and molecular toxicity of lead in primary and clonal osteoblastic bone cells. United States: N. p., 1989. Web.
Long, G J. The cellular and molecular toxicity of lead in primary and clonal osteoblastic bone cells. United States.
Long, G J. 1989. "The cellular and molecular toxicity of lead in primary and clonal osteoblastic bone cells". United States.
@article{osti_6703681,
title = {The cellular and molecular toxicity of lead in primary and clonal osteoblastic bone cells},
author = {Long, G J},
abstractNote = {First, steady state kinetic models of lead metabolism and calcium homeostasis were developed in both primary and clonal osteoblastic bone cells. Secondly, the effect of lead on cellular calcium homeostasis was determined. Finally, the effect of lead on 1,25 (OH){sub 2}D{sub 3} induced production of osteocalcin, a protein synthesized and secreted by osteoblasts, was investigated. Lead metabolism in osteoblastic bone cells was characterized by three intracellular pools. The largest of these, S{sub 3}, included mitochondrial lead and accounted for 70 percent of total cell lead in primary osteoblastic bone cells and 85 percent of total lead in clonal osteoblastic bone cells. None of the kinetic pools were saturated at lead concentrations up to 100 {mu}M lead. Calcium homeostasis in osteoblastic bone cells was also described by a three compartment, intracellular kinetic model.},
doi = {},
url = {https://www.osti.gov/biblio/6703681}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Sun Jan 01 00:00:00 EST 1989},
month = {Sun Jan 01 00:00:00 EST 1989}
}

Miscellaneous:
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