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Title: Lysophosphatidic acid synthesis and phospholipid metabolism in rat mast cells

The role of lysophosphatidic acid in mast cell response to antigen was investigated using an isolated rat serosal mast cell model. The cells were incubated with monoclonal murine immunoglobulin E to the dinitrophenyl hapten and prelabeled with /sup 32/P-orthophosphate or /sup 3/H-fatty acids. Lysophosphatidic acid was isolated form cell extracts by 2-dimensional thin-layer chromatography, and the incorporated radioactivity was assessed by liquid scintillation counting. Lysophosphatidic acid labeling with /sup 32/P was increased 2-4 fold within 5 minutes after the addition of antigen or three other mast cell agonists. Functional group analyses unequivocally showed that the labeled compound was lysophosphatidic acid. Lysophosphatidic acid synthesis was dependent on the activity of diacylglycerol lipase, suggesting formation from monoacylglycerol. In addition, the studies of lysophosphatidic acid synthesis suggest that the addition of antigen to mast cells may initiate more than one route of phospholipid degradation and resynthesis. Whatever the origin of lysophosphatidic acid, the results of this study demonstrated that lysophosphatidic acid synthesis is stimulated by a variety of mast cell agonists. Dose-response, kinetic, and pharmacologic studies showed close concordance between histamine release and lysophosphatidic acid labeling responses. These observations provide strong evidence that lysophosphatidic acid plays an important role in mast cell activation.
Authors:
Publication Date:
OSTI Identifier:
6703340
Resource Type:
Book
Resource Relation:
Other Information: Thesis
Publisher:
University of Texas Health Science Center,Dallas, TX
Research Org:
Texas Univ., Houston (USA). Health Science Center
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CARBOXYLIC ACIDS; BIOSYNTHESIS; MAST CELLS; CHEMICAL ACTIVATION; PHOSPHOLIPIDS; METABOLISM; ANTIGENS; BIOLOGICAL MODELS; DOSE-RESPONSE RELATIONSHIPS; IMMUNOGLOBULINS; PHOSPHATES; PHOSPHORUS 32; RATS; SCINTILLATION COUNTING; THIN-LAYER CHROMATOGRAPHY; TRITIUM COMPOUNDS; ANIMAL CELLS; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CHROMATOGRAPHY; CONNECTIVE TISSUE CELLS; COUNTING TECHNIQUES; DAYS LIVING RADIOISOTOPES; ESTERS; GLOBULINS; ISOTOPES; LABELLED COMPOUNDS; LIGHT NUCLEI; LIPIDS; MAMMALS; NUCLEI; ODD-ODD NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC PHOSPHORUS COMPOUNDS; OXYGEN COMPOUNDS; PHOSPHORUS COMPOUNDS; PHOSPHORUS ISOTOPES; PROTEINS; RADIOISOTOPES; RODENTS; SEPARATION PROCESSES; SOMATIC CELLS; SYNTHESIS; VERTEBRATES 550201* -- Biochemistry-- Tracer Techniques