Determination of ( sup 3 H)-2,3,7,8-tetrachlorodibenzo-p-dioxin in human feces to ascertain its relative metabolism in man

Journal Article · Sun Apr 15 00:00:00 EDT 1990 · Analytical Chemistry (Washington); (USA)

DOI:https://doi.org/10.1021/ac00207a005· OSTI ID:6702969

Wendling, J M; Orth, R G; Poiger, H ^[1]

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1. Monsanto Company, St. Louis, MO (USA)

Human feces samples from a self-dosing experiment were analyzed by gas chromatography/mass spectrometry (GC/MS) for ({sup 3}H)-2,3,7,8-tetrachlorodibenzo-p-dioxin ({sup 3}H-2378-TCDD) to determine that 36-44% of the radioactivity was attributable to the parent compound. This method, using isotope dilution analysis, proved to be difficult due to the unexpectedly higher native 2378-TCDD background which created abnormally large precision ranges around the calculated feces concentrations of 0.1-0.2 pg/g. These results were supported by additional analyses involving the GC/MS chemical cleanup method combined with liquid scintillation counting which showed that at most, 50% of the radioactivity was due to 2378-TCDD metabolites resulting in a minimum metabolism of 50% for these samples.

• OSTI ID: 6702969
• Journal Information: Analytical Chemistry (Washington); (USA), Vol. 62:8; ISSN 0003-2700
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
DIOXIN
METABOLISM
FECES
GAS CHROMATOGRAPHY
MAN
MASS SPECTROSCOPY
METABOLITES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMALS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
CHROMATOGRAPHY
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
MAMMALS
MATERIALS
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PRIMATES
SEPARATION PROCESSES
SPECTROSCOPY
VERTEBRATES
WASTES
560300* - Chemicals Metabolism & Toxicology
550501 - Metabolism- Tracer Techniques