Characterization and immunotherapeutic potential of a monoclonal antibody against a ras oncogene transformed cell line

Thesis/Dissertation · Wed Jan 01 00:00:00 EST 1986

OSTI ID:6701131

Ames, Jr, R S

Transformed cells express cell surface antigens not present, or present in diminished amounts on normal cells. Monoclonal antibodies can be used to identify and biochemically characterize tumor-associated antigens. Monoclonal antibody (MoAb) 45-2D9 was produced by immunization of BALB/c mice with a transformed cell line (45-2D9) induced by transfection of NIH 3T3 cells with a c-H-ras oncogene in DNA isolated from a human lung carcinoma. By immunoperoxidase staining, this antibody binds to the 45-342 cells as well as to the ras transformed primary and 3 secondary transfectants, including the one used to induce 45-342, but not to other ras transformed cell lines. Murine tumors as well as human fetal and most normal adult tissues are not stained. This antibody does bind to a variety of human tumors, including lung adenocarcinomas, as well as breast, colon and esophageal carcinomas. The ability of MoAb 45-2D9 to target ricin toxin A chain (RTA) and radio-isotopes to gp74 expressing cells was investigated. An immunotoxin generated by conjugating RTA to MoAb 45-2D9 inhibits protein and DNA synthesis by the 45-342 cells. Radiolabeled antibody specifically localizes to and can be used to image subcutaneous and pulmonary gp74 expressing tumors in nu/nu mice. Monoclonal antibodies against oncogene transformed cell lines may be useful for the detection and characterization of tumor-associated antigens as well as for the development of new tumor therapeutic and diagnostic reagents.

• Research Organization: George Washington Univ., Washington, DC (USA)
• OSTI ID: 6701131
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
CARCINOMAS
IMMUNOTHERAPY
LABELLED COMPOUNDS
DIAGNOSTIC USES
MONOCLONAL ANTIBODIES
ANTIGENS
DNA REPLICATION
ESOPHAGUS
FIBROBLASTS
IODINE 125
LUNGS
MAMMARY GLANDS
MICE
ONCOGENES
ONCOGENIC TRANSFORMATIONS
TUMOR CELLS
ANIMAL CELLS
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BODY
CELL TRANSFORMATIONS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
GENES
GLANDS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
MAMMALS
NEOPLASMS
NUCLEI
NUCLEIC ACID REPLICATION
ODD-EVEN NUCLEI
ORGANS
RADIOISOTOPES
RESPIRATORY SYSTEM
RODENTS
SOMATIC CELLS
THERAPY
USES
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics