GABA and benzodiazepine receptors in the gerbil brain after transient ischemia: demonstration by quantitative receptor autoradiography

Journal Article · Sun Feb 01 00:00:00 EST 1987 · J. Cereb. Blood Flow Metab.; (United States)

DOI:https://doi.org/10.1038/jcbfm.1987.12· OSTI ID:6696229

Onodera, H; Sato, G; Kogure, K

Quantitative receptor autoradiography was used to measure the binding of gamma-aminobutyric acid (GABA) and benzodiazepine receptors after ischemia by means of transient occlusion of bilateral common carotid arteries in the gerbil. (/sup 3/H)Muscimol was used to label the GABAA receptors and (/sup 3/H)flunitrazepam to label central type benzodiazepine receptors. In the superolateral convexities of the frontal cortices, (/sup 3/H)muscimol binding was increased in 60% of the animals killed 3 days after ischemia, and decreased in 67% of the animals killed 27 days after ischemia. Twenty-seven days after ischemia, (/sup 3/H)flunitrazepam binding in the substantia nigra pars reticulata increased to 252% of the control, though the increase in (/sup 3/H)muscimol binding was not significant. In the dorsolateral region of the caudate putamen, marked neuronal necrosis and depletion of both (/sup 3/H)muscimol and (/sup 3/H)flunitrazepam binding sites were observed 27 days after ischemia, the ventromedial region being left intact. In spite of the depletion of pyramidal cells in the CA1 region of the hippocampus, both (/sup 3/H)muscimol and (/sup 3/H)flunitrazepam binding sites were preserved 27 days after ischemia. Since our previous study revealed that adenosine A1 binding sites were depleted in the CA1 subfield of the hippocampus after ischemia correlating with neuronal damage, GABAA and benzodiazepine receptors may not be distributed predominantly on the pyramidal cells in the CA1 region.

• Research Organization: Tohoku Univ. School of Medicine, Sendai, Japan
• OSTI ID: 6696229
• Journal Information: J. Cereb. Blood Flow Metab.; (United States), Vol. 1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
AMINOBUTYRIC ACID
RADIORECEPTOR ASSAY
BRAIN
AUTORADIOGRAPHY
CENTRAL NERVOUS SYSTEM DEPRESSANTS
HIPPOCAMPUS
ISCHEMIA
RECEPTORS
RODENTS
TRITIUM COMPOUNDS
AMINO ACIDS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BODY
CARBOXYLIC ACIDS
CARDIOVASCULAR DISEASES
CENTRAL NERVOUS SYSTEM
DISEASES
DRUGS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
NERVOUS SYSTEM
NEUROREGULATORS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
TRACER TECHNIQUES
VASCULAR DISEASES
VERTEBRATES
551001* - Physiological Systems- Tracer Techniques