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Title: Inhibitory action of sphingosine, sphinganine and dexamethasone on glucose uptake: Studies with hydrogen peroxide and phorbol ester

Abstract

The mechanism of the inhibitory action of glucocorticoids on glucose uptake is incompletely understood. Treatment with corticosteriods of cells in which glucose uptake is stimulated at insulin postbinding and postreceptor sites may clarify the site of the steroid inhibitory action. Hydrogen peroxide, which has been shown to stimulate the insulin receptor tyrosine kinase, and phorbol myristate acetate (PMA) which stimulates protein kinase C were, therefore, used as stimulators of glucose transport in this study. These studies demonstrate that dexamethasone and the sphingoid bases, sphinganine and sphingosine, inhibit glucose uptake that has been stimulated at either the receptor kinase or protein kinase C level in both 3T3-L1 and 3T3-C2 cells. These data confirm glucocorticoid inhibitory action at a post binding level and support the suggestion that some corticosteriod inhibitory effects may be mediated by an action on sphingolipid metabolism.

Authors:
; ;  [1]
  1. Univ. of Utah School of Medicine, Salt Lake City (USA)
Publication Date:
OSTI Identifier:
6695426
Resource Type:
Journal Article
Journal Name:
Life Sciences; (USA)
Additional Journal Information:
Journal Volume: 46:25; Journal ID: ISSN 0024-3205
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; GLUCOCORTICOIDS; BIOCHEMICAL REACTION KINETICS; GLUCOSE; UPTAKE; HYDROGEN PEROXIDE; BIOLOGICAL EFFECTS; PHORBOL ESTERS; DEXAMETHASONE; INHIBITION; INSULIN; PHOSPHOTRANSFERASES; RECEPTORS; ADRENAL HORMONES; ALDEHYDES; CARBOHYDRATES; CARCINOGENS; CORTICOSTEROIDS; ENZYMES; ESTERS; HEXOSES; HORMONES; HYDROGEN COMPOUNDS; HYDROXY COMPOUNDS; KETONES; KINETICS; MEMBRANE PROTEINS; MONOSACCHARIDES; ORGANIC COMPOUNDS; OXYGEN COMPOUNDS; PEPTIDE HORMONES; PEROXIDES; PHOSPHORUS-GROUP TRANSFERASES; PREGNANES; PROTEINS; REACTION KINETICS; SACCHARIDES; STEROIDS; TRANSFERASES; 560300* - Chemicals Metabolism & Toxicology

Citation Formats

Murray, D K, Hill, M E, and Nelson, D H. Inhibitory action of sphingosine, sphinganine and dexamethasone on glucose uptake: Studies with hydrogen peroxide and phorbol ester. United States: N. p., 1990. Web. doi:10.1016/0024-3205(90)90236-K.
Murray, D K, Hill, M E, & Nelson, D H. Inhibitory action of sphingosine, sphinganine and dexamethasone on glucose uptake: Studies with hydrogen peroxide and phorbol ester. United States. https://doi.org/10.1016/0024-3205(90)90236-K
Murray, D K, Hill, M E, and Nelson, D H. 1990. "Inhibitory action of sphingosine, sphinganine and dexamethasone on glucose uptake: Studies with hydrogen peroxide and phorbol ester". United States. https://doi.org/10.1016/0024-3205(90)90236-K.
@article{osti_6695426,
title = {Inhibitory action of sphingosine, sphinganine and dexamethasone on glucose uptake: Studies with hydrogen peroxide and phorbol ester},
author = {Murray, D K and Hill, M E and Nelson, D H},
abstractNote = {The mechanism of the inhibitory action of glucocorticoids on glucose uptake is incompletely understood. Treatment with corticosteriods of cells in which glucose uptake is stimulated at insulin postbinding and postreceptor sites may clarify the site of the steroid inhibitory action. Hydrogen peroxide, which has been shown to stimulate the insulin receptor tyrosine kinase, and phorbol myristate acetate (PMA) which stimulates protein kinase C were, therefore, used as stimulators of glucose transport in this study. These studies demonstrate that dexamethasone and the sphingoid bases, sphinganine and sphingosine, inhibit glucose uptake that has been stimulated at either the receptor kinase or protein kinase C level in both 3T3-L1 and 3T3-C2 cells. These data confirm glucocorticoid inhibitory action at a post binding level and support the suggestion that some corticosteriod inhibitory effects may be mediated by an action on sphingolipid metabolism.},
doi = {10.1016/0024-3205(90)90236-K},
url = {https://www.osti.gov/biblio/6695426}, journal = {Life Sciences; (USA)},
issn = {0024-3205},
number = ,
volume = 46:25,
place = {United States},
year = {Mon Jan 01 00:00:00 EST 1990},
month = {Mon Jan 01 00:00:00 EST 1990}
}