skip to main content

Title: Novel actinomycete and a cyanide-degrading pseudomonad isolated from industrial sludge

A novel actinomycete was the predominant filamentous microorganism in bulking activated sludge in a bench-scale reactor treating coke plant wastewater. The bacterium was isolated and identified as an actinomycete that is biochemically and morphologically similar to Amycolatopsis orientalis; however, a lack of DNA homology excludes true relatedness. At present, the isolate (NRRL B 16216) cannot be assigned to the recognized taxa of actinomycetes. Cyanide-degrading microorganisms were selected in chemostats maintained at a low dilution rate for several weeks. Cyanide alone or cyanide plus phenol were fully degraded when equilibrium was achieved, and increasing concentrations of cyanide were degraded until inhibition of cell division resulted in cell washout. An isolated non-fluorescent pseudomonad could be adapted to degrade high concentrations of cyanide and to utilize cyanide-nitrogen when phenol or lactate was the carbon source. Although one-carbon compounds such as methanol and methylamine were growth substrates, cyanide was not utilized as a carbon source. In the absence of cyanide, adaptation was gradually lost. Oxygen consumption of adapted cells was stimulated in the presence of cyanide whereas that of unadapted cells was depressed. Cyanide was degraded by growing or resting cells and by cell-free extracts. Cyanide degrading activity of cell-free extracts, lost upon dialysis,more » was fully restored with NAD(P)H.« less
Authors:
Publication Date:
OSTI Identifier:
6688368
Resource Type:
Thesis/Dissertation
Resource Relation:
Other Information: Thesis (Ph. D.)
Publisher:
Univ. of Alabama,Birmingham, AL
Research Org:
Alabama Univ., Birmingham (USA)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 01 COAL, LIGNITE, AND PEAT; ACTIVATED SLUDGE PROCESS; MICROORGANISMS; CYANIDES; BIODEGRADATION; WASTE WATER; WATER TREATMENT; BACTERIA; BATCH CULTURE; BENCH-SCALE EXPERIMENTS; BIOLOGICAL ADAPTATION; COKING PLANTS; INHIBITION; PHENOL; AROMATICS; CHEMICAL REACTIONS; DECOMPOSITION; HYDROGEN COMPOUNDS; HYDROXY COMPOUNDS; INDUSTRIAL PLANTS; LIQUID WASTES; MANAGEMENT; ORGANIC COMPOUNDS; OXYGEN COMPOUNDS; PHENOLS; PROCESSING; WASTE MANAGEMENT; WASTE PROCESSING; WASTES; WATER 550700* -- Microbiology; 010800 -- Coal, Lignite, & Peat-- Waste Management