Novel fermentation: the production of R(-)-1,2-propanediol and acetol by Clostridium thermosaccharolyticum

Journal Article · Tue Jul 01 00:00:00 EDT 1986 · Bio/Technology; (United States)

DOI:https://doi.org/10.1038/nbt0786-651· OSTI ID:6663689

Cameron, D C; Cooney, C L

Three strains of Clostridium thermosaccharolyticum were found that produce R(-)-1,2-propanediol from a variety of sugars, including D-glucose and D-xylose. The fermentation of glucose by strain HG-8 (ATCC 31960) gave 7.9 g/l of R(-)-1,2-propanediol with a best yield of 0.27 g/g glucose and an enantiomeric excess of greater than 99%. Acetol accumulated to 1.47 g/l. Product formation was not affected by phosphate concentrations up to 113 mM. A possible pathway to these products involves a variation of the methylglyoxal bypass. Methylglyoxal is reduced to acetol, which is further reduced to %(-)-1,2-propanediol. This fermentation provides a unique route to R(-)-1,2-propanediol and acetol from inexpensive, readily available substrates.

• Research Organization: Massachusetts Institute of Technology, Cambridge
• DOE Contract Number: AC02-83CH10093
• OSTI ID: 6663689
• Journal Information: Bio/Technology; (United States), Vol. 4:7
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
CLOSTRIDIUM THERMOSACCHAROLYTICUM
PRODUCTIVITY
GLUCOSE
FERMENTATION
HYDROXY ACIDS
BIOSYNTHESIS
XYLOSE
ADDITIVES
BIOLOGICAL PATHWAYS
FOOD PROCESSING
ALDEHYDES
BACTERIA
BIOCONVERSION
CARBOHYDRATES
CARBOXYLIC ACIDS
CLOSTRIDIUM
HEXOSES
MICROORGANISMS
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PENTOSES
PROCESSING
SACCHARIDES
SYNTHESIS
140504* - Solar Energy Conversion- Biomass Production & Conversion- (-1989)