Physical mapping withing the tuberous sclerosis linkage group in region 9q32-q34

Harris, R M; Carter, N P; Griffiths, B; Goudie, D; Hampson, R M; Yates, J R.W.; Affara, N A; Ferguson-Smith, M A

doi:10.1006/geno.1993.1056

Title: Physical mapping withing the tuberous sclerosis linkage group in region 9q32-q34

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Abstract

Pulsed-field gel electrophoresis and flow dot-blot analysis have been used to construct a physical map of the q32-q34 region of chromosome 9, where one of the loci responsible for tuberous sclerosis (TSC1) has been mapped by genetic linkage. Five linked groups of markers have been defined by pulsed-field gel electrophoresis. The orientation of these groups and the order of markers within them were determined by hybridization to flow-sorted dot blots derived from a panel of cell lines of chromosome 9 translocations to place probes proximal or distal to each breakpoint. The local map order 9q32-q34 derived by application of this combination of techniques is as follows: centromere - ALAD-1.3 Mb-ORM/20 kb/D9S16-GSN-250 kb-C5-HXB-1.9 Mb-D9S21-AK1-1.4 Mb-SPTAN1-ASS-800-kb-ABL-2 Mb-D0S10/350 Kb/DBH-telomere. 48 refs., 6 figs., 4 figs.

Authors:

Harris, R M; Carter, N P; Griffiths, B; Goudie, D; Hampson, R M; Yates, J R.W.; Affara, N A; Ferguson-Smith, M A ^[1]

Univ. of Cambridge (United Kingdom)

Publication Date:: Mon Feb 01 00:00:00 EST 1993

OSTI Identifier:: 6660432

Resource Type:: Journal Article

Journal Name:: Genomics; (United States)

Additional Journal Information:: Journal Volume: 15:2; Journal ID: ISSN 0888-7543

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; HEREDITARY DISEASES; GENETIC MAPPING; HUMAN CHROMOSOMES; ELECTROPHORESIS; HYBRIDIZATION; ORIENTATION; PROBES; CHROMOSOMES; DISEASES; MAPPING; 550400* - Genetics

Citation Formats


                    Harris, R M, Carter, N P, Griffiths, B, Goudie, D, Hampson, R M, Yates, J R.W., Affara, N A, and Ferguson-Smith, M A. Physical mapping withing the tuberous sclerosis linkage group in region 9q32-q34.  United States: N. p., 1993. 
        Web.  doi:10.1006/geno.1993.1056.

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                    Harris, R M, Carter, N P, Griffiths, B, Goudie, D, Hampson, R M, Yates, J R.W., Affara, N A, & Ferguson-Smith, M A. Physical mapping withing the tuberous sclerosis linkage group in region 9q32-q34.  United States.  https://doi.org/10.1006/geno.1993.1056

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                    Harris, R M, Carter, N P, Griffiths, B, Goudie, D, Hampson, R M, Yates, J R.W., Affara, N A, and Ferguson-Smith, M A. 1993.  
        "Physical mapping withing the tuberous sclerosis linkage group in region 9q32-q34".  United States.  https://doi.org/10.1006/geno.1993.1056.

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@article{osti_6660432,

  title        = {Physical mapping withing the tuberous sclerosis linkage group in region 9q32-q34},

  author       = {Harris, R M and Carter, N P and Griffiths, B and Goudie, D and Hampson, R M and Yates, J R.W. and Affara, N A and Ferguson-Smith, M A},

  abstractNote = {Pulsed-field gel electrophoresis and flow dot-blot analysis have been used to construct a physical map of the q32-q34 region of chromosome 9, where one of the loci responsible for tuberous sclerosis (TSC1) has been mapped by genetic linkage. Five linked groups of markers have been defined by pulsed-field gel electrophoresis. The orientation of these groups and the order of markers within them were determined by hybridization to flow-sorted dot blots derived from a panel of cell lines of chromosome 9 translocations to place probes proximal or distal to each breakpoint. The local map order 9q32-q34 derived by application of this combination of techniques is as follows: centromere - ALAD-1.3 Mb-ORM/20 kb/D9S16-GSN-250 kb-C5-HXB-1.9 Mb-D9S21-AK1-1.4 Mb-SPTAN1-ASS-800-kb-ABL-2 Mb-D0S10/350 Kb/DBH-telomere. 48 refs., 6 figs., 4 figs.},

  doi          = {10.1006/geno.1993.1056},

  url          = {https://www.osti.gov/biblio/6660432},
  journal      = {Genomics; (United States)},

  issn         = {0888-7543},

  number       = ,

  volume       = 15:2,

  place        = {United States},

  year         = {Mon Feb 01 00:00:00 EST 1993},

  month        = {Mon Feb 01 00:00:00 EST 1993}

}

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