skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Two-compartment behavior during transport of folate compounds in L1210 cell plasma membrane vesicles

Abstract

The transport of (/sup 3/H) 1,L 5-formyltetrahydrofolate, (/sup 3/H) folic acid, and (/sup 3/H)methotrexate by L1210 cell plasma membrane vesicles exhibited multicompartmental behavior. Two separate vesicular compartments (parallel relationship) of approximately equal volume were revealed during measurements of influx and efflux. Flux in one compartment was rapid, saturable, highly temperature-sensitive, and inhibited by pCMBS. Flux in the other compartment exhibited all of the characteristics of passive diffusion. These results imply that our plasma membrane vesicle preparations consist of a mixture of two functional species. Transport of folate into one of these species occurs by passive diffusion alone, whereas transport into the other kind of vesicle occurs by both passive diffusion and carrier-facilitated transport.

Authors:
; ;
Publication Date:
Research Org.:
Memorial Sloan-Kettering Cancer Center, New York, New York
OSTI Identifier:
6657251
Resource Type:
Journal Article
Journal Name:
J. Membr. Biol.; (United States)
Additional Journal Information:
Journal Volume: 68:1
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; FOLIC ACID; PHARMACOLOGY; METHOTREXATE; TRITIUM COMPOUNDS; TRACER TECHNIQUES; BIOCHEMICAL REACTION KINETICS; CELL MEMBRANES; COMPARTMENTS; LEUKEMIA; PERMEABILITY; AMINO ACIDS; ANTIMETABOLITES; CARBOXYLIC ACIDS; CELL CONSTITUENTS; DISEASES; DRUGS; HEMATINICS; HEMATOLOGIC AGENTS; HEMIC DISEASES; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; ISOTOPE APPLICATIONS; KINETICS; LABELLED COMPOUNDS; MEMBRANES; NEOPLASMS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PTERIDINES; REACTION KINETICS; VITAMIN B GROUP; VITAMINS; 550201* - Biochemistry- Tracer Techniques; 551001 - Physiological Systems- Tracer Techniques; 550901 - Pathology- Tracer Techniques

Citation Formats

Yang, C H, Dembo, M, and Sirotnak, F M. Two-compartment behavior during transport of folate compounds in L1210 cell plasma membrane vesicles. United States: N. p., 1982. Web. doi:10.1007/BF01872250.
Yang, C H, Dembo, M, & Sirotnak, F M. Two-compartment behavior during transport of folate compounds in L1210 cell plasma membrane vesicles. United States. https://doi.org/10.1007/BF01872250
Yang, C H, Dembo, M, and Sirotnak, F M. 1982. "Two-compartment behavior during transport of folate compounds in L1210 cell plasma membrane vesicles". United States. https://doi.org/10.1007/BF01872250.
@article{osti_6657251,
title = {Two-compartment behavior during transport of folate compounds in L1210 cell plasma membrane vesicles},
author = {Yang, C H and Dembo, M and Sirotnak, F M},
abstractNote = {The transport of (/sup 3/H) 1,L 5-formyltetrahydrofolate, (/sup 3/H) folic acid, and (/sup 3/H)methotrexate by L1210 cell plasma membrane vesicles exhibited multicompartmental behavior. Two separate vesicular compartments (parallel relationship) of approximately equal volume were revealed during measurements of influx and efflux. Flux in one compartment was rapid, saturable, highly temperature-sensitive, and inhibited by pCMBS. Flux in the other compartment exhibited all of the characteristics of passive diffusion. These results imply that our plasma membrane vesicle preparations consist of a mixture of two functional species. Transport of folate into one of these species occurs by passive diffusion alone, whereas transport into the other kind of vesicle occurs by both passive diffusion and carrier-facilitated transport.},
doi = {10.1007/BF01872250},
url = {https://www.osti.gov/biblio/6657251}, journal = {J. Membr. Biol.; (United States)},
number = ,
volume = 68:1,
place = {United States},
year = {Fri Jan 01 00:00:00 EST 1982},
month = {Fri Jan 01 00:00:00 EST 1982}
}