Antibiotic bonding to polytetrafluoroethylene with tridodecylmethylammonium chloride
Polytetrafluoroethylene (PTFE) treated with the cationic surfactant, triodecylmethylammonium chloride (TDMAC), binds /sup 14/C-penicillin (1.5 to 2 mg antibiotic/cm graft), whereas untreated PTFE or PTFE treated with anionic detergents shows little binding of antibiotic. TDMAC-treated PTFE concomitantly binds penicillin and heparin, generating a surface that potentially can resist both infection and thrombosis. The retention of these biologically active molecules is not due to passive entrapment in the PTFE but reflects an ionic interaction between the anionic ligands and surface-bound TDMAC. Penicillin bound to PTFE is not removed by exhaustive washing in aqueous buffers but is slowly released in the presence of plasma or when the PTFE is placed in a muscle pouch in the rat. Muscle tissue adjacent to the treated PTFE shows elevated levels of antibiotic following implantation. PTFE treated with TDMAC and placed in a muscle pouch binds /sup 14/C-penicillin when it is locally irrigated with antibiotic or when penicillin is administered intravenously. Thus, the TDMAC surface treated either in vitro or in vivo with penicillin provides an effective in situ source for the timed release of antibiotic.
- Research Organization:
- Department of Biochemistry and Surgery, UMD-Rutgers Medical School, Piscataway, NJ
- OSTI ID:
- 6656731
- Journal Information:
- Surgery; (United States), Vol. 92:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
CARBON 14 COMPOUNDS
TRACER TECHNIQUES
HEPARIN
PHARMACOLOGY
PENICILLIN
BIOCHEMICAL REACTION KINETICS
CHEMICAL PREPARATION
RESPONSE MODIFYING FACTORS
SURFACTANTS
AMINES
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTICOAGULANTS
CARBOHYDRATES
DRUGS
HEMATOLOGIC AGENTS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MUCOPOLYSACCHARIDES
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
POLYSACCHARIDES
REACTION KINETICS
SACCHARIDES
SYNTHESIS
550601* - Medicine- Unsealed Radionuclides in Diagnostics
550201 - Biochemistry- Tracer Techniques