Lung-derived growth factors: possible paracrine effectors of fetal lung development

Thesis/Dissertation · Tue Jan 01 00:00:00 EST 1985

OSTI ID:6655162

Montes, A M

A potential role for paracrine secretions in lung organogenesis has been hypothesized (Alescio and Piperno, 1957). These studies present direct support for the paracrine model by demonstrating the presence of locally produced mitogenic/maturational factors in fetal rat lung tissue. Conditioned serum free medium (CSFM) from nineteen-day fetal rat lung cultures was shown to contain several bioactive peptides as detected by /sup 3/H-Thymidine incorporation into chick embryo and rat lung fibroblasts, as well as /sup 14/C-choline incorporation into surfactant in mixed cell cultures. Using ion-exchange chromatography and Sephadex gel filtration, a partially purified mitogen, 11-III, was obtained. The partially purified 11-III stimulates mitosis in chick embryo fibroblasts and post-natal rat lung fibroblasts. Multiplication in fetal rat lung fibroblasts cultures is stimulated only when these are pre-incubated with a competence factor or unprocessed CSFM. This suggests the existence of an endogenously produced competence factor important in the regulation of fetal lung growth. Preparation 11-III does not possess surfactant stimulating activity as assessed by /sup 3/H-choline incorporation into lipids in predominantly type-II cell cultures. These data demonstrate the presence of a maturational/mitogenic factor, influencing type-II mixed cell cultures. In addition, 11-III had been shown to play an autocrine role stimulating the proliferation of fetal lung fibroblasts. Finally, these data suggest the existence of a local produced competence factor.

• Research Organization: Hawaii Univ., Honolulu (USA)
• OSTI ID: 6655162
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CHOLINE
UPTAKE
GROWTH
STIMULATION
LUNGS
MORPHOLOGICAL CHANGES
RATS
FETUSES
THYMIDINE
CARBON 14 COMPOUNDS
CELL CULTURES
FIBROBLASTS
GEL PERMEATION CHROMATOGRAPHY
ION EXCHANGE CHROMATOGRAPHY
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ALCOHOLS
AMINES
AMMONIUM COMPOUNDS
ANIMAL CELLS
ANIMALS
AZINES
BODY
CHROMATOGRAPHY
CONNECTIVE TISSUE CELLS
DRUGS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPOTROPIC FACTORS
MAMMALS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRIMIDINES
QUATERNARY COMPOUNDS
RESPIRATORY SYSTEM
RIBOSIDES
RODENTS
SEPARATION PROCESSES
SOMATIC CELLS
VERTEBRATES
550301* - Cytology- Tracer Techniques
550801 - Morphology- Tracer Techniques