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Title: Lung-derived growth factors: possible paracrine effectors of fetal lung development

A potential role for paracrine secretions in lung organogenesis has been hypothesized (Alescio and Piperno, 1957). These studies present direct support for the paracrine model by demonstrating the presence of locally produced mitogenic/maturational factors in fetal rat lung tissue. Conditioned serum free medium (CSFM) from nineteen-day fetal rat lung cultures was shown to contain several bioactive peptides as detected by /sup 3/H-Thymidine incorporation into chick embryo and rat lung fibroblasts, as well as /sup 14/C-choline incorporation into surfactant in mixed cell cultures. Using ion-exchange chromatography and Sephadex gel filtration, a partially purified mitogen, 11-III, was obtained. The partially purified 11-III stimulates mitosis in chick embryo fibroblasts and post-natal rat lung fibroblasts. Multiplication in fetal rat lung fibroblasts cultures is stimulated only when these are pre-incubated with a competence factor or unprocessed CSFM. This suggests the existence of an endogenously produced competence factor important in the regulation of fetal lung growth. Preparation 11-III does not possess surfactant stimulating activity as assessed by /sup 3/H-choline incorporation into lipids in predominantly type-II cell cultures. These data demonstrate the presence of a maturational/mitogenic factor, influencing type-II mixed cell cultures. In addition, 11-III had been shown to play an autocrine role stimulating the proliferation ofmore » fetal lung fibroblasts. Finally, these data suggest the existence of a local produced competence factor.« less
Authors:
Publication Date:
OSTI Identifier:
6655162
Resource Type:
Thesis/Dissertation
Resource Relation:
Other Information: Thesis (Ph. D.)
Publisher:
Univ. of Hawaii,Honolulu, HI
Research Org:
Hawaii Univ., Honolulu (USA)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CHOLINE; UPTAKE; GROWTH; STIMULATION; LUNGS; MORPHOLOGICAL CHANGES; RATS; FETUSES; THYMIDINE; CARBON 14 COMPOUNDS; CELL CULTURES; FIBROBLASTS; GEL PERMEATION CHROMATOGRAPHY; ION EXCHANGE CHROMATOGRAPHY; TRACER TECHNIQUES; TRITIUM COMPOUNDS; ALCOHOLS; AMINES; AMMONIUM COMPOUNDS; ANIMAL CELLS; ANIMALS; AZINES; BODY; CHROMATOGRAPHY; CONNECTIVE TISSUE CELLS; DRUGS; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; LIPOTROPIC FACTORS; MAMMALS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; PYRIMIDINES; QUATERNARY COMPOUNDS; RESPIRATORY SYSTEM; RIBOSIDES; RODENTS; SEPARATION PROCESSES; SOMATIC CELLS; VERTEBRATES 550301* -- Cytology-- Tracer Techniques; 550801 -- Morphology-- Tracer Techniques