DNA sequence of 15 base pairs is sufficient to mediate both glucocorticoid and progesterone induction of gene expression
To define the recognition sequence of the glucocorticoid receptor and its relationship with that of the progesterone receptor, oligonucleotides derived from the glucocorticoid response element of the tyrosine aminotransferase gene were tested upstream of a heterologous promoter for their capacity to mediate effects of these two steroids. The authors show that a 15-base-pair sequence with partial symmetry is sufficient to confer glucocorticoid inducibility on the promoter of the herpes simplex virus thymidine kinase gene. The same 15-base-pair sequence mediates induction by progesterone. Point mutations in the recognition sequence affect inducibility by glucocorticoids and progesterone similarly. Together with the strong conservation of the sequence of the DNA-binding domain of the two receptors, these data suggest that both proteins recognize a sequence that is similar, if not the same.
- Research Organization:
- Institute of Cell and Tumor Biology, Heidelberg (Germany, F.R.)
- OSTI ID:
- 6651978
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:22
- Country of Publication:
- United States
- Language:
- English
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GLUCOCORTICOIDS
GENE REGULATION
PROGESTERONE
TRANSFERASES
AUTORADIOGRAPHY
DNA SEQUENCING
MAMMARY GLANDS
MAN
MESSENGER-RNA
OLIGONUCLEOTIDES
PHOSPHORUS 32
RECEPTORS
TRANSCRIPTION
TUMOR CELLS
ADRENAL HORMONES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CORTICOSTEROIDS
DAYS LIVING RADIOISOTOPES
ENZYMES
GLANDS
HORMONES
HYDROXY COMPOUNDS
ISOTOPES
KETONES
LIGHT NUCLEI
MAMMALS
MEMBRANE PROTEINS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS ISOTOPES
PREGNANES
PRIMATES
PROTEINS
RADIOISOTOPES
RNA
STEROID HORMONES
STEROIDS
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques