Shedding of mitotic cells from the surface of multicell spheroids during growth
During the growth of EMT6/Ro mammary tumor multicell spheroids, a large number of cells are shed into the suspension medium. The rate of cell shedding was 218 cells per square millimeter of spheroid surface per hour, or up to 1.5% of the total spheroid cell content per hour. Shed cells had a clonogenic capacity equal to that of exponential monolayer cultures and were further characterized by volume distribution, mitotic index, flow cytofluorometry, and autoradiography. The results indicated that cells are released from the spheroid surface at mitosis, presumably due to a loosening of the cell-to-cell attachment during this cycle phase. These mitotic cells, when placed in monolayer culture, attached and grew synchronously with a cell cycle time of about 13 hours. Shed cells kept in suspension culture had a similar cell cycle time, but these cells reaggregated immediately after mitosis. The results indicated that cell shedding and reaggregation both occur near the time of mitosis and are intrinsic factors regulating the initiation and subsequent growth of multicell spheroids. Although these studies were done with spheroids cultured in vitro, shedding of mitotic cells may play an important role in the in vivo process of metastasis.
- Research Organization:
- Univ. of Rochester, NY
- DOE Contract Number:
- DE-AC02-76EV03490
- OSTI ID:
- 6644106
- Journal Information:
- J. Cell. Physiol.; (United States), Vol. 106:1
- Country of Publication:
- United States
- Language:
- English
Similar Records
Heterogeneity in multicell spheroids induced by alterations in the external oxygen and glucose concentration
Rate of death of hypoxic cells in multicell spheroids. [Effects of hypoxia on survival and radiosensitivity of spheroids; hypoxic cells of which are models of cells within solid tumors]
Related Subjects
MITOSIS
BIOLOGICAL EFFECTS
SPHEROIDS
GROWTH
BUILDUP
CELL CULTURES
CELL CYCLE
CELL PROLIFERATION
CYTOLOGY
DATA
DNA
HISTOLOGICAL TECHNIQUES
IN VITRO
TIME DEPENDENCE
TUMOR CELLS
ANIMAL CELLS
BIOLOGY
CELL DIVISION
INFORMATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
550300* - Cytology