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Title: Schistosoma mansoni: vaccination of mice with 10-krad-irradiated, cryopreserved schistosomules

Protection against a Schistosoma mansoni cercarial challenge was evaluated in mice immunized with a vaccine composed of 10-krad-irradiated, cryopreserved schistosomules. The level of resistance induced in C57B1/6 or NMRI (CV) mice increased with the number of schistosomules injected. Up to 83% reduction in challenge worm burden was achieved when 5000 schistosomules were injected per mouse. Intramuscular injection of the vaccine was superior to subcutaneous. Multiple immunizations, up to 3 at 4-week intervals, did not increase the resistance induced by a single immunization. A high level of protection developed in as little as 2 weeks and was maintained through at least 12 weeks postimmunization. The vaccine irradiated with 10 krad from either a 60-cobalt or 137-cesium source induced equivalent levels of resistance, and no differences were found in the immunogenicity of vaccines comprised of organisms irradiated as cercariae or as 1- to 3-hr-old schistosomules. These findings are basic to the development of a cryopreserved, live vaccine against schistosomiasis of humans or domestic animals.
Authors:
; ;
Publication Date:
OSTI Identifier:
6628989
Resource Type:
Journal Article
Resource Relation:
Journal Name: Exp. Parasitol.; (United States); Journal Volume: 57:3
Research Org:
Biomedical Research Inst., Rockville, MD
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; SCHISTOSOMA; FREEZING; INFECTIVITY; RADIOPRESERVATION; VACCINES; CHEMICAL PREPARATION; TESTING; CESIUM 137; COBALT 60; CRYOBIOLOGY; INOCULATION; MICE; SCHISTOSOMIASIS; ALKALI METAL ISOTOPES; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGY; CESIUM ISOTOPES; COBALT ISOTOPES; DISEASES; HELMINTHS; INFECTIOUS DISEASES; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; IRRADIATION; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; MAMMALS; MINUTES LIVING RADIOISOTOPES; NUCLEI; ODD-EVEN NUCLEI; ODD-ODD NUCLEI; PARASITIC DISEASES; PLATYHELMINTHS; PRESERVATION; RADIOISOTOPES; RODENTS; SYNTHESIS; TREMATODES; VERTEBRATES; YEARS LIVING RADIOISOTOPES 560134* -- Radiation Effects on Microorganisms-- Vaccine Preparation & Other Applications-- (-1987); 551000 -- Physiological Systems; 070203 -- Radiation Sources-- Use in Medical Supply Sterilization-- (-1987)