Juvenile hormone biosynthesis and secretion by the female Corpora allata of the larval gypsy moth, Lymantria dispar (L. ) utilizing in vitro organ culture

Thesis/Dissertation · Wed Jan 01 00:00:00 EST 1986

OSTI ID:6619169

Jones, G L

Junvenile hormone synthesis and secretion in the female larval gypsy moth was investigated. In vitro culturing methods were developed including: incubating 2 pair of CC-CA gland complexes in 50 ul of osmotically balanced Grace's insect medium containing 1 uCi /sup 3/H-methyl-methionine for 6 hr. JH homologues were identified and quantified using TLC and HPLC. In vitro methods were employed to investigate trends of JH secretion in 4th and ultimate female larval instar CA. Fourth instar CA produced JH peaks of 0.15 pmole/pr/hr between days 2 and 3, but the rate declined to half by day 4. Ultimate instar larvae began secreting 0.48 pmole/pr/hr, but by day 10, had decreased JH output to negligible levels which continued until pupation. Effects upon in vitro JH secretion produced by precocene II and caffeine were examined. Feulgen staining techniques revealed an equal number of cells (30) in 4th and last instar CA. Last instar Ca were 3 times larger than 4th in volume but their actual in vitro JH secretion at peak levels was only 20% greater. In vitro methods demonstrated that JH secretory trends differ in younger versus mature larval instars. Glandular volume increased in last instars but JH secretion was only 20% greater than in 4th's when compared on the basis of volume. Precocene II elicited a negative response on in vivo JH secretion at levels 10 times less than caffeine. Caffeine was judged not to significantly alter JH secretion.

• Research Organization: Massachusetts Univ., Amherst (USA)
• OSTI ID: 6619169
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
HORMONES
BIOSYNTHESIS
SECRETION
LYMANTRIA DISPAR
BIOCHEMISTRY
CAFFEINE
LARVAE
LIQUID COLUMN CHROMATOGRAPHY
METHIONINE
THIN-LAYER CHROMATOGRAPHY
TISSUE CULTURES
TRITIUM COMPOUNDS
AMINO ACIDS
ANALEPTICS
ANIMALS
AROMATICS
ARTHROPODS
AZAARENES
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM AGENTS
CHEMISTRY
CHROMATOGRAPHY
DRUGS
HETEROCYCLIC COMPOUNDS
INSECTS
INVERTEBRATES
LABELLED COMPOUNDS
LEPIDOPTERA
LIPOTROPIC FACTORS
MOTHS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PURINES
SEPARATION PROCESSES
SYNTHESIS
XANTHINES
550201* - Biochemistry- Tracer Techniques